Human p53 circRNA
p53 is an important tumor suppressor protein that maintains genome integrity by regulating cell cycle, promoting DNA repair, inducing apoptosis and inhibiting tumorigenesis. When a cell is subjected to DNA damage or other stress signals, the p53 protein is activated and accumulates in the nucleus, initiating a series of gene expression programs. These programs can halt the cell cycle, give cells time to repair DNA damage, or induce apoptosis when the damage is irreparable, thereby preventing the proliferation of damaged cells. Mutations in the p53 gene are one of the most common genetic changes in a variety of cancers, resulting in a loss of function that causes cells to lose control of proliferation, which in turn promotes the occurrence and development of tumors. Therefore, p53 is of great significance in cancer research and treatment and has become an important target of targeted therapy.
Human p53 circRNA provides a concentration of about 1 mg/mL. Circular RNA has a unique ring structure and lacks 5' and 3' ends, which makes it more stable in the cell, not easily degraded by exonuclease, and can continuously and stably express human P53 protein for a long time.
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Concentration |
~1 mg/mL |
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Buffer |
RNase free water |
Storage |
-40°C or below |
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General tips |
Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. |
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Shipping Condition |
ship with dry ice |
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It can be based on your experimental goals:
- For Tracking Transfection and Translation Efficiency: APExBIO Reporter Gene mRNAs (e.g. EGFP, Firefly Luciferase mRNA) are commonly used to track transfection efficiency and protein expression duration; evaluate gene expression and cell viability; study mRNA localization and bio-distribution via in vivo imaging; optimize transfection conditions and validate LNP delivery system.
- For Gene Editing, Functional Studies and Gene Therapy Research: APExBIO offers various functional protein mRNAs, involving tumor suppressors (e.g. p53, PTEN), cytokines (e.g. IL-12, IL-10), gene-editing tools (e.g. spCas9, Cre Recombinase), gene replacement protein (e.g. EPO), and antigens (e.g. OVA, SARS-CoV-2 Spike).
- For Sustained Protein Expression: APExBIO Self-amplifying RNA (saRNA) and Circular RNA (circRNA) are recommended for applications requiring prolonged protein expression. saRNA enables lasting and strong protein expression at lower doses, while circRNA has enhanced structural stability and extended expression duration.
- Advanced Capping Technology: Utilizes Cap 1 structure (EZ Cap™ Cap) to achieve enhanced translation efficiency and minimizing activation of the host innate immune response. The capping efficiency can reach 90–99%.
- Diverse Modification Options: Provides a range of modified nucleotides, such as m1Ψ B8049, 5-moUTP B8061 and Cy5-UTP B8333, which reduce immunogenicity, improve mRNA stability, and maximize protein expression levels.
- Stringent Quality Control: Each batch undergoes rigorous quality assessment including capping efficiency, purity, integrity, and sterility to ensure batch-to-batch consistency.
