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Catalog No.
Intracellular ratiometric pH indicator
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Ship with 5-10 days
Ship with 5-10 days
Ship with 5-10 days

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BCECF-AM is a fluorescent dye that can penetrate cell membranes. It is a commonly used fluorescent probe to detect intracellular pH[1]. 

BCECF-AM has no fluorescence. After entering the cell, it is hydrolyzed by intracellular esterase to BCECF, and is left in the cell. BCECF can be excited to form green fluorescence at the appropriate pH[2]. Intracellular pH measurement is performed by ratio imaging, which is the ratio of the emission intensity (detected at 535 nm) of the dye when excited at 490 nm to 440 nm[1,3]. 

BCECF-AM is not only widely used in the study of mammalian cells, but also has been reported for the detection of intracellular pH levels in animal tissues, plant cells, bacteria and yeast. BCECF AM is widely used in processes such as cytotoxicity, apoptosis, cell adhesion, drug resistance, and chemotaxis with changes in intracellular pH. 


[1]. Ozkan P, and Mutharasan R. A rapid method for measuring intracellular pH using BCECF-AM. Biochimica et Biophysica Acta, 2002, 1572(1): 143-148 . 

[2]. Dive C, Cox H, Watson J V, et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol.Cell Probe, 1988,  2(2): 131-145. 

[3]. O'Connor N, Silver R B. Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol, 2007, 81: 415-433 . 

[4]. Chow S, Hedley D, and Tannock I. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases. Cytometry, 1996, 24: 360-367.

Product Citation

Chemical Properties

Physical AppearanceA yellow film
StorageStore at -20°C
Cas No.117464-70-7
SolubilitySoluble in DMSO
Chemical Namebis(acetoxymethyl) 3,3'-(3',6'-bis(acetoxymethoxy)-5-((acetoxymethoxy)carbonyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)dipropanoate
SDFDownload SDF
Canonical SMILESO=C1O[[email protected]@]2(C3=C1C=C(C(OCOC(C)=O)=O)C=C3)C(C=C(CCC(OCOC(C)=O)=O)C(OCOC(C)=O)=C4)=C4OC5=C2C=C(CCC(OCOC(C)=O)=O)C(OCOC(C)=O)=C5
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure