In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BCECF-AM is a fluorescent dye that can penetrate cell membranes. It is a commonly used fluorescent probe to detect intracellular pH.
BCECF-AM has no fluorescence. After entering the cell, it is hydrolyzed by intracellular esterase to BCECF, and is left in the cell. BCECF can be excited to form green fluorescence at the appropriate pH. Intracellular pH measurement is performed by ratio imaging, which is the ratio of the emission intensity (detected at 535 nm) of the dye when excited at 490 nm to 440 nm[1,3].
BCECF-AM is not only widely used in the study of mammalian cells, but also has been reported for the detection of intracellular pH levels in animal tissues, plant cells, bacteria and yeast. BCECF AM is widely used in processes such as cytotoxicity, apoptosis, cell adhesion, drug resistance, and chemotaxis with changes in intracellular pH.
. Ozkan P, and Mutharasan R. A rapid method for measuring intracellular pH using BCECF-AM. Biochimica et Biophysica Acta, 2002, 1572(1): 143-148 .
. Dive C, Cox H, Watson J V, et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol.Cell Probe, 1988, 2(2): 131-145.
. O'Connor N, Silver R B. Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol, 2007, 81: 415-433 .
. Chow S, Hedley D, and Tannock I. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases. Cytometry, 1996, 24: 360-367.
|Physical Appearance||A yellow film|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||bis(acetoxymethyl) 3,3'-(3',6'-bis(acetoxymethoxy)-5-((acetoxymethoxy)carbonyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)dipropanoate|
|Canonical SMILES||O=C1O[[email protected]@]2(C3=C1C=C(C(OCOC(C)=O)=O)C=C3)C(C=C(CCC(OCOC(C)=O)=O)C(OCOC(C)=O)=C4)=C4OC5=C2C=C(CCC(OCOC(C)=O)=O)C(OCOC(C)=O)=C5|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|