ATAC-Seq Library Preparation Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ATAC-Seq (Assay for Transposase-Accessible Chromatin with High-throughput Sequencing) is a new method developed for mapping chromatin accessibility genome-wide. The method is used to infer regions of increased accessibility, as well as to map regions of transcription/regulation factor binding and nucleosome position. The purpose of experiments is answering deep questions about regulation of gene expression through detection of promoters, enhancers, or other regulatory elements binding regions, characterizing relationships between cis-regulatory elements and non-coding genetic variation.
Our product ATAC-Seq Library Preparation Kit provides a simple, convenient and time-saving tool for you to analyze epigenetic information of your cells or tissues. The kit includes a hyperactive Tn5 transposase, which fragments the DNA in open regions of chromatin and tags the DNA with sequencing adapters simultaneously. The adapters are compatible with the Illumina sequencing platform, so you can obtain the nucleotide base sequence after the workflow. 20-30 mg tissue or 50,000-100,000 cells are applicable for each reaction.
| Components | 24 rxns | 96 rxns | |
| Box 1 | ATAC Lysis Buffer | 30 mL | 120 mL |
| 10% Tween 20 | 50 μL | 200 μL | |
| 10% NP40 | 50 μL | 200 μL | |
| Transposase Mix | 130 μL | 500 μL | |
| Box 2 | Digitonin | 20 mg | 20 mg | 2X Tagmentation Buffer | 800 μL | 3 mL |
| Amplify Enzyme | 30 μL | 100 μL | |
| 5X Amplify Buffer | 250 μL | 1 mL | |
| dNTP Mixture (10 mM) | 30 μL | 100 μL | |
Box 1: Store at 4°C. Box 2: Store at -20°C. The Index (Cat. No. K1058 and K1059) used for PCR is supplied with two specification. For Digitonin solution, please avoid repeated freeze/thaw cycles as possible, and it can be kept at -20°C for up to 6 months. | |||
Assess the epigenetic profile of open chromatin regions more conveniently compared to DNase-Seq or FAIRE-Seq
Simple and rapid Four-step protocol
Sensitive for characterizing specific cell populations
