MagBeads Plasmid Mini Preparation Kit
Alkaline lysis method separates chromosomal DNA from plasmid DNA based on differences in DNA denaturation and renaturation, making it a widely used method for plasmid DNA preparation. The magnetic bead-based plasmid purification kit uses an improved SDS alkaline lysis method combined with a dual magnetic bead-based plasmid DNA extraction technique. This approach enables stable, efficient, and convenient plasmid DNA extraction. The kit avoids the need for high-speed centrifugation or vacuum filtration, allowing for rapid removal of impurities and specific binding of plasmid DNA.
This kit is suitable for extracting plasmid DNA from 1-50 mL overnight cultures of E. coli and can be used for downstream molecular biology experiments such as DNA sequencing, PCR, cell transfection, in vitro transcription, and restriction enzyme digestion. It supports both manual operation and automated extraction using nucleic acid extraction instruments for high-throughput automation.
|
Components |
K1628-20Tests |
K1628-100Tests |
Storage |
||
|
Buffer 1 |
5 mL |
25mL |
Room Temperature |
||
|
Buffer 2 |
5 mL |
25 mL |
Room Temperature |
||
|
Buffer 3 |
7 mL |
35 mL |
Room Temperature |
||
|
Buffer B1 |
3.5 mL |
17.5 mL |
Room Temperature |
||
|
MagBeads |
3 mL |
15 mL |
4℃ |
||
|
Buffer W1 |
6 mL |
30 mL |
Room Temperature |
||
|
Buffer EB |
2 mL |
10 mL |
Room Temperature |
||
|
RNase A |
0.1 mL |
0.5 mL |
-20℃ |
||
|
Shipping: Blue Ice Shelf life: 12 months |
|||||