In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IOX 1 (5-carboxy-8HQ) is an inhibitor of histone demethylase JMJD with IC50 values of 0.2 μM for JMJD2E .
The Jumonji domain (JMJD) is a histone demethylase and acts as a transcriptional repressor.
IOX 1 is a histone demethylase JMJD inhibitor. In the FDH-coupled assay, IOX 1 inhibited JMJD2E with IC50 value of 0.2 μM. In MALDI-TOF MS assay, IOX 1 inhibited JMJD2E, JMJD2A, FIH and PHD2 with IC50 values of 2.4, 1.7, 20.5 and 14.3 μM, respectively. In HeLa cells, IOX 1 increased H3K9me3 fluorescence intensity, suggesting that IOX 1 inhibited H3K9me3 demethylation induced by JMJD2A with IC50 value of 86.5 μM. Also, IOX 1 exhibited cytotoxicity with IC50 value of 291.6 μM .
. King ON, Li XS, Sakurai M, et al. Quantitative high-throughput screening identifies 8-hydroxyquinolines as cell-active histone demethylase inhibitors. PLoS One, 2010, 5(11): e15535.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥5.75mg/mL in DMSO|
|Chemical Name||8-hydroxyquinoline-5-carboxylic acid|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Description||IOX1 is a potent and broad-spectrum inhibitor of 2OG oxygenases.|