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Hydrocortisone

Catalog No.
B1951
steroid hormone or glucocorticoid
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$55.00
In stock
1g
$50.00
In stock
5g
$80.00
In stock

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Background

Hydrocortisone is a main glucocorticoid secreted by the adrenal cortex.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt362.46
Cas No.50-23-7
FormulaC21H30O5
Solubility≥13.3mg/mL in DMSO
Chemical Name(8S,9S,10R,11S,13S,14S,17R)-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-2,6,7,8,9,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-3-one
SDFDownload SDF
Canonical SMILESCC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1]:

Cell lines

human lung microvascular endothelial cells

Preparation method

The solubility of this compound in DMSO is >13.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

4 or 6 μM; 16 h

Applications

In human lung microvascular endothelial cells, Hydrocortisone (HC) induced a concentration-dependent but biologically minor barrier enhancing and protective effect. The combination of HC (4 μM) and ascorbic acid (VitC) (1000 μM) pre- or post-treatment significantly reversed the LPS-induced barrier dysfunction.

Animal experiment [2]:

Animal models

6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD) mouse models

Dosage form

0.4 mg/kg; intraperitoneal injection, 7 consecutive days

Application

In Parkinson’s disease (PD) mouse models, Hydrocortisone caused approximate two-fold increase of parkin expression in selected brain subregions (striatum, ventral midbrain, and cerebellum), which was responsible for cell survival against oxidative stress.. Hydrocortisone also increased CREB expression. Hydrocortisone pretreatment markedly enhanced dopaminergic neuronal survival against 6-OHDA intoxication.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Barabutis N1, Khangoora V2, Marik PE2, et al. Hydrocortisone and Ascorbic Acid synergistically prevent and repair LPS- induced pulmonary endothelial barrier dysfunction. Chest. 2017 Jul 21. pii: S0012-3692(17)31276-X.

[2]. Ham S1, Lee YI2,3, Jo M1, et al. Hydrocortisone-induced parkin prevents dopaminergic cell death via CREB pathway in Parkinson's disease model. Sci Rep. 2017 Apr 3;7(1):525.

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