Tautomycetin
Tautomycetin is a potent, selective inhibitor of PP1, with IC50 values being 1.6 nM and 62 nM for the PP1 and PP2A subtypes, respectively. PP1, one of four major protein serine/threonine phosphatases (PPs), plays an important role in regulation of many cellular events such as transcription, translation, cell division, muscle contraction, glycogen synthesis, and neuronal signaling. Compared with over 40 species of natural phosphatase inhibitors reported, tautomycetin is the most specific PP1 inhibitor, which can be served as a novel powerful tool to elucidate the physiological roles of PP1 in a variety of biological events.
Reference:
1. Mitsuhashi S, Matsuura N, Ubukata M, et al. Tautomycetin is a novel and specific inhibitor of serine/threonine protein phosphatase type 1, PP1. Biochemical and Biophysical Research Communications, 2001, 287(2): 328-331.
| Physical Appearance | Colourless liquid |
| Storage | Desiccate at -20°C |
| M.Wt | 606.75 |
| Cas No. | 119757-73-2 |
| Formula | C33H50O10 |
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 10mg/ml) |
| Chemical Name | (S)-(2S,3S,4R,7S,8R,11R,13R,Z)-17-ethyl-4,8-dihydroxy-3,7,11,13-tetramethyl-6,15-dioxononadeca-16,18-dien-2-yl 3-hydroxy-3-(4-methyl-2,5-dioxo-2,5-dihydrofuran-3-yl)propanoate |
| SDF | Download SDF |
| Canonical SMILES | O[C@@H]([C@H](C)C(C[C@H]([C@H](C)[C@H](C)OC(C[C@@H](C1=C(C)C(OC1=O)=O)O)=O)O)=O)CC[C@@H](C)C[C@@H](C)CC(/C=C(C=C)/CC)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
Human primary T cells |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
1 μg/ml for 5 h |
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Applications |
Tautomycetin possesses immunosuppressive activity to inhibit the proliferation of T cells by inhibition of IL-2 secretion. |
| Animal experiment [1]: | |
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Animal models |
Rats after heterotopic cardiac transplantation |
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Dosage form |
0.05 mg/kg. Tautomycetin solubilized in PBS or in microemulsion form for 30 days after heterocardiac transplantation. |
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Application |
Immunosuppressive activity of tautomycetin is effective in an in vivo organ transplantation model (rats after heterotopic cardiac transplantation). |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Jae-Hyuck Shim, et al. Immunosuppressive effects of tautomycetin in vivo and in vitro via T cell-specific apoptosis induction. Proc Natl Acad Sci U S A. 2002 Aug 6; 99(16): 10617–10622. | |
Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
