Protein A (Liquid form)
Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. The recombinant Protein A is a genetically engineered protein containing five IgG-binding regions of protein A and 6×His-tag on N-terminus and C-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6×His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
Quality Control & DataSheet
- View current batch:
Purity: ≥99%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
|Gene ID:||3919448||Accession #:||YP_498670|
|Alternate Names:||Protein A, rProtein A, r-protein A, recombinant protein A, IgG purification, antibody purification, Protein A beads, Protein A sepharose, Protein A Sepharose Beads, proteina, protein-A, protein a, protein/a.|
|Source:||Escherichia Coli.||M.Wt:||39.21 kDa|
|Appearance:||Liquid||Storage:||Store at -20℃|
|Formulation:||50 mg/ml in H2O.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|