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Protein A (Liquid form)

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Protein A, used for purification of IgG antibodies, expressed in E.Coli.
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Protein A is a 42 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus. It can bind immunoglobulins. The recombinant Protein A is a genetically engineered protein containing five IgG-binding regions of protein A and 6×His-tag on N-terminus and C-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6×His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.

Quality Control

Quality Control & DataSheet

View current batch:
    Purity: ≥99%, by SDS-PAGE and HPLC.
    Endotoxin Level:<0.1 ng/μg


Gene ID: 3919448 Accession #: YP_498670
Alternate Names:Protein A, rProtein A, r-protein A, recombinant protein A, IgG purification, antibody purification, Protein A beads, Protein A sepharose, Protein A Sepharose Beads, proteina, protein-A, protein a, protein/a.
Source: Escherichia Coli. M.Wt: 39.21 kDa
Appearance: Liquid Storage: Store at -20℃
Formulation:50 mg/ml in H2O.
Shipping Condition: Gel pack. Handling: Centrifuge the vial prior to opening.
Usage:For Research Use Only! Not to be used in humans.