Mouse IFN gamma ELISPOT Kit

IFN-γ (interferon-γ) is a type II interferon family cytokine involved in antiviral, antibacterial, and antitumor responses. In addition, IFN-γ acts as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-γ dimers signal through a receptor complex of two IFN-γ R1 and two IFN-γ R2 subunits.

Enzyme-linked immunospot (ELISPOT) technology is the best technology in the world to detect the level of cellular immunity in organisms. It combines high sensitivity, high confidence, high throughput, single-cell level, functional assays, and low cost. This ELISpot assay uses a capture antibody specific for mouse IFN-γ that is pre-coated onto a PVDF-supported microplate. Appropriately stimulated cells are pipetted directly into the wells, and immobilized antibodies near secreting cells are bound to secreted mouse IFN-γ. After the washing step and incubation with the biotinylated detection antibody, horseradish oxidase conjugated to streptavidin is added. The unbound enzymes are subsequently removed by washing and a substrate solution (AEC solution) is added. A red precipitate is formed at the cytokine localization site and appears as spots, each individual spot representing a single mouse IFN-γ secretory cell, and finally counted using an automated ELISpot reader system.