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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Mirin is a potent MRN complex inhibitor. Mirin inhibits Mre11-associated exonuclease activity, rather than alters DNA-binding or MRN complex formation. Moreover, mirin prevents MRN-dependent activation of ATM in response to DNA double-strand breaks, with an IC50 value of 12 μM. The MRN complex acts as a DNA damage sensor, responsible for maintaining genome stability during DNA replication, promoting homology-dependent DNA repair and activating ATM. The MRN-ATM pathway plays an essential role in sensing and signaling from DNA double-strand breaks.
Reference:
1. Dupré A, Boyer-Chatenet L, Sattler RM, et al. A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nature Chemical Biology, 2008, 4(2): 119-125.
Cell lines
Human embryonic kidney (HEK)293 cells and TOSA4 cells
Reaction Conditions
10 ~ 100 μM mirin for 24 h incubation
Applications
In mammalian cells, mirin induced G2 arrest, abolished the radiation-induced G2/M checkpoint, and prevented homology-directed repair of DNA damage.
Note
The technical data provided above is for reference only.
References: