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Mirin

Catalog No.
B5389
A potent inhibitor of the Mre11-Rad50-Nbs1 (MRN)-ATM pathway
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$99.00
In stock
10mg
$91.00
In stock
50mg
$251.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Mirin is a potent MRN complex inhibitor. Mirin inhibits Mre11-associated exonuclease activity, rather than alters DNA-binding or MRN complex formation. Moreover, mirin prevents MRN-dependent activation of ATM in response to DNA double-strand breaks, with an IC50 value of 12 μM. The MRN complex acts as a DNA damage sensor, responsible for maintaining genome stability during DNA replication, promoting homology-dependent DNA repair and activating ATM. The MRN-ATM pathway plays an essential role in sensing and signaling from DNA double-strand breaks. 

Reference:

1. Dupré A, Boyer-Chatenet L, Sattler RM, et al. A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nature Chemical Biology, 2008, 4(2): 119-125.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt220.25
Cas No.1198097-97-0
FormulaC10H8N2O2S
Solubilityinsoluble in EtOH; insoluble in H2O; ≥9.3 mg/mL in DMSO
Chemical Name(Z)-5-(4-hydroxybenzylidene)-2-imino-2,5-dihydrothiazol-4-ol
SDFDownload SDF
Canonical SMILESN=C1N=C(O)/C(S1)=C([H])/C2=CC=C(O)C=C2
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

Human embryonic kidney (HEK)293 cells and TOSA4 cells

Reaction Conditions

10 ~ 100 μM mirin for 24 h incubation

Applications

In mammalian cells, mirin induced G2 arrest, abolished the radiation-induced G2/M checkpoint, and prevented homology-directed repair of DNA damage.

Note

The technical data provided above is for reference only.

References:

1. Dupré A, Boyer-Chatenet L, Sattler RM, et al. A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nature Chemical Biology, 2008, 4(2): 119-125.

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