Batimastat (BB-94)
Batimastat (BB-94; CAS: 130370-60-4) is a synthetic small-molecule inhibitor targeting matrix metalloproteinases (MMPs). Structurally a polypeptide-like analogue of collagen substrates, batimastat contains a peptidic backbone and a hydroxamate moiety that binds the catalytic zinc atom of MMPs. It inhibits several MMP subtypes markedly, including MMP-1, MMP-2, MMP-3, MMP-7, and MMP-9, with reported IC50 values of 3, 4, 20, 6, and 4 nM, respectively. In preclinical studies, batimastat demonstrates inhibitory effects on tumor growth and angiogenesis across various tumor models, including ovarian and colon carcinoma xenografts, making it relevant for cancer research and therapeutic development.
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| Physical Appearance | A solid |
| Storage | Store at 4°C |
| M.Wt | 477.64 |
| Cas No. | 130370-60-4 |
| Formula | C23H31N3O4S2 |
| Synonyms | Batimastat, BB-94 |
| Solubility | ≥23.88 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH |
| Chemical Name | (2S,3R)-N1-hydroxy-3-isobutyl-N4-((S)-1-(methylamino)-1-oxo-3-phenylpropan-2-yl)-2-((thiophen-2-ylthio)methyl)succinamide |
| SDF | Download SDF |
| Canonical SMILES | CNC([C@@H](NC([C@@H]([C@H](CSC1=CC=CS1)C(NO)=O)CC(C)C)=O)CC2=CC=CC=C2)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment: | |
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Cell lines |
C170HM2 and AP5LV cell lines |
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Preparation method |
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes and/or shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months. |
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Reaction Conditions |
96 h; 3.0 μg/mL |
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Applications |
In vitro proliferation in the presence of batimastatwas assessed by the MTT absorbance assay. The effect of batimastat on the in vitro growth of C170HM2 and AP5LV in both serum-free and scrum-containing culture media was determined three times. Batimastat had no significant effect on the growth of either cell line. |
| Animal experiment: | |
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Animal models |
Orthotopic transplant model of human colon cancer in nude mice |
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Dosage form |
30 mg/kg; i.p. |
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Applications |
Treatment with BB-94 caused a significant reduction in the median weight of the primary tumor from 293 mg (range, 1141 to 124 mg) in the control group to 144 mg (range, 424 to 38 mg) in the BB-94 treated group (P <0.001) and resulted in a marked reduction in the incidence of tumor invasion of adjacent tissue, from 12 of 18 mice in the control group (67%) to 7 of 20 mice in the BB-94 treated group (35%). |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] Watson S A, Morris T M, Robinson G, et al. Inhibition of organ invasion by the matrix metalloproteinase inhibitor batimastat (BB-94) in two human colon carcinoma metastasis models [J]. Cancer research, 1995, 55 (16): 3629-3633. [2] Wang X, Fu X, Brown P D, et al. Matrix metalloproteinase inhibitor BB-94 (batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice [J]. Cancer research, 1994, 54 (17): 4726-4728. |
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| Description | Batimastat (BB-94) is a potent, broad spectrum inhibitor of matrix metalloprotease (MMP) for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. | |||||
| Targets | MMP-1 | MMP-2 | MMP-9 | MMP-7 | MMP-3 | |
| IC50 | 3 nM | 4 nM | 4 nM | 6 nM | 20 nM | |
Quality Control & MSDS
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