Pyridostatin

Grouped product items
Size	Price	Stock
10mM (in 1mL DMSO)	Please inquire	Please inquire
5mg	Please inquire	Please inquire
10mg	Please inquire	Please inquire
25mg	Please inquire	Please inquire
50mg	Please inquire	Please inquire

Pyridostatin (CAS No. 1085412-37-8) is a synthetic G‑quadruplex stabilizer that can accommodate the dynamic and structurally diverse nature of G‑quadruplexes. Pyridostatin competitively binds against telomere‑associated proteins and induces telomere dysfunction.

The free‑base form of this product is highly unstable and readily degrades, so in experimental settings it is commonly used in the form of its TFA salt, Pyridostatin TFA (Catalog No.: A9061, CAS No.: 1472611‑44‑1).

1. Emily G Oldani, Kevin M Reynolds Caicedo, et al. "The effect of G - quadruplexes on TDP43 condensation, distribution, and toxicity." Structure. 2025 May 27:S0969-2126(25)00184-4. PMID: 40480222
2. Zhenzhen Yan, Axin He, et al. "Structural Insights into an Antiparallel Chair‐Type G‐Quadruplex From the Intron of NOP56 Oncogene." Adv Sci (Weinh). 2025 Apr;12(16):e2406230. PMID: 40047221
3. Emily G. Oldani, Kevin M. Reynolds Caicedo, et al. "Manipulating TDP43 Aggregation via RNA G-quadruplexes." bioRxiv. April 30, 2024
4. Sowers ML, Conrad JW, et al. "DNA Base Excision Repair Intermediates Influence Duplex–Quadruplex Equilibrium." Molecules 2023 Jan 18;28(3) PMID: 36770637
5. Vivek M Shastri, Veena Subramanian, et al. "A novel cell-cycle-regulated interaction of the Bloom syndrome helicase BLM with Mcm6 controls replication-linked processes." Nucleic Acids Res. 2021 Sep 7;49(15):8699-8713. PMID: 34370039
6. Vlasenok M, Varizhuk A, et al. "Data on secondary structures and ligand interactions of G-rich oligonucleotides that defy the classical formula for G4 motifs." Data Brief. 2017 Feb 12;11:258-265 PMID: 28243622
7. Varizhuk A, Ischenko D, et al. "The expanding repertoire of G4 DNA structures." Biochimie. 2017 Apr;135:54-62 PMID: 28109719

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Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	596.64
Cas No.	1085412-37-8
Formula	C31H32N8O5
Synonyms	RR-82;RR82;RR 82
Solubility	≥20.85 mg/mL in DMSO; ≥30.87 mg/mL in EtOH with gentle warming; ≥9.66 mg/mL in H2O with gentle warming and ultrasonic
Chemical Name	4-(2-aminoethoxy)-2-N,6-N-bis[4-(2-aminoethoxy)quinolin-2-yl]pyridine-2,6-dicarboxamide
Canonical SMILES	C1=CC=C2C(=C1)C(=CC(=N2)NC(=O)C3=CC(=CC(=N3)C(=O)NC4=NC5=CC=CC=C5C(=C4)OCCN)OCCN)OCCN
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Cell experiment [1]:
Cell lines	HeLa, HT1080, U2OS and WI-38 cell lines
Preparation method	The solubility of this compound in DMSO is >20.85 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition	0–40 μM for 72 h
Applications	A previous study investigated the growth inhibition after 3 days of exposure to pyridostatin on a panel of four human cell lines: HeLa (adenocarcinoma), HT1080 (fibrosarcoma), U2OS (osteosarcoma), and WI-38 (normal lung fibroblasts), the latter being non-cancerous. Pyridostatin showed growth inhibition at high nanomolar to low micromolar concentrations against these tested cell lines. In addition, pyridostatin exhibited an 18.5-fold selectivity for HT1080 cells over WI-38 cells.
References: [1] Müller S, Sanders D A, Di Antonio M, et al. Pyridostatin analogues promote telomere dysfunction and long-term growth inhibition in human cancer cells. Organic & biomolecular chemistry, 2012, 10(32): 6537-6546.