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Indirubin

Indirubin

Catalog No.

B3672

cyclin-dependent kinases and GSK-3β inhibitor

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Grouped product items
Size	Price	Stock
10mM (in 1mL DMSO)	$50.00	In stock
5mg	$40.00	In stock
25mg	$180.00	In stock
100mg	$470.00	In stock
250mg	$680.00	In stock

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Background
Chemical Properties
Protocol
Quality Control

Background

Interferon-γ is thought to be a member of the inflammatory cytokine family. Because of its augmentation of inflammatory reactions through biological activities. Indirubin, the active ingredient of Danggui Longhui Wan, has inhibitory effects on the production of interferon-γ.

In vitro: Indirubin exerted its inhibitory effects not only on interferon-γ production by human myelomonocytic HBL-38 cells but also on interferon-γ and interleukin-σ production by murine splenocytes with no influence on the proliferation of either cells [1].

In vivo: Because of Indirubin’s inhibitory activity on interferon-γ production, the authors further investigated the effects of indirubin on 2,4,6-trinitro-l-chlorobenzene-induced delayed-type hypersensitivity. When injected intraperitoneally, indirubin significantly inhibited the ear swelling of TNCB-elicited mice. Moreover,the amount of interferon-γ in the culture supernatants of elicited mouse lymphocytes was inhibited by indirubin treatment [1].

Clinical trials: Several clnical trials have been conducted to study the efficacy of indirubin for the treatments of psoriasis and Acute Promyeloid Leukemia .

Reference:
[1] Kunikata T, Tatefuji T, Aga H, Iwaki K, Ikeda M, Kurimoto M. Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. Eur J Pharmacol. 2000 Dec 20;410(1):93-100.

Chemical Properties

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	262.26
Cas No.	479-41-4
Formula	C₁₆H₁₀N₂O₂
Solubility	Soluble in DMSO > 10 mM
SDF	Download SDF
Canonical SMILES	O=C1/C(NC2=CC=CC=C21)=C3C4=CC=CC=C4NC\3=O
Shipping Condition	Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tips	For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1]:
Cell lines	HBL-38 and HPB-ALL cells
Preparation method	The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.
Reacting condition	0 ~ 1000 ng/mL; for 24 hrs
Applications	In HBL-38 cells, Indirubin (10 ng/mL) strongly inhibited interferon-γ production without any influence on cell proliferation. The IC50 value of Indirubin was 0.15 ng/mL. However, in HPB-ALL cells, Indirubin did not affect interleukin-4 production stimulated by phytohemaggulutinin, as well as cell proliferation.
Animal experiment [1]:
Animal models	TNCB-induced delayed-type hypersensitivity mouse model
Dosage form	20 mg/day; i.p.; 4 days before sensitization to 4 days after sensitization
Applications	In the single elicitation experiment, Indirubin significantly inhibited ear swelling of the TNCB-induced delayed-type hypersensitivity mouse model at the 24th hr after treatment. In the experiment with repeated epicutaneous applications, Indirubin also inhibited ear swelling but these effects gradually reduced. Meanwhile, Indirubin did not cause any body weight loss.
Other notes	Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References: [1]. Kunikata T, Tatefuji T, Aga H, Iwaki K, Ikeda M, Kurimoto M. Indirubin inhibits inflammatory reactions in delayed-type hypersensitivity. Eur J Pharmacol. 2000 Dec 20;410(1):93-100.