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His-tag Protein Purification Kit (Reductant and Chelator Resistant)

Catalog No.
K4206
His-tag Protein Purification Kit
Grouped product items
SizePriceStock Qty
10ml
$100.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

The His-tag Protein Purification Kit extracts His-tagged recombinant proteins from cells/lysates using affinity media (e.g., Ni-Agarose, Ni-NTA) that bind histidine residues. Target proteins are adsorbed, impurities are washed off, and targets are eluted via buffer changes. Tolerating reducing agents (e.g., 20 mM DTT) and chelators (e.g., 20 mM EDTA) but incompatible with 8 M urea or 6 M guanidine hydrochloride (requiring dialysis for inclusion body proteins), it enables 10 purifications (≈1g bacterial pellet each) with max 10-15 mg total protein (3-10 mg for 50 kD proteins) or 500 small-scale purifications without columns.

Quality Control

Quality Control & DataSheet

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Related Biological Data

His-tag Protein Purification Kit (Reductant and Chelator Resistant)
 

Components and Storage

Components

10 mL

Storage

His-tag Purification Resin (Reductant and Chelator Resistant)

10 mL

4℃

Native Lysis Buffer

120 mL

4℃

Native Wash Buffer

60 mL

4℃

Native Elution Buffer

60 mL

4℃

Lysozyme

60 mg

4℃

Empty Affinity Chromatography Column (3 mL)

10 Sets

4℃

DTT (Solid)

10X 1 Tube

-20℃

Shipping: Blue Ice                                 Shelf life: 12 months