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Caspase-3, human recombinant proteinase

Catalog No.
E1003
Caspase-3, cysteine proteinase, expressed in E.Coli.
Grouped product items
SizePriceStock Qty
25 units
$145.00
Ship with 10-15 days
100 units
$309.00
Ship with 10-15 days
5ug
$334.00
Ship with 10-15 days
10ug
$561.00
Ship with 10-15 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Caspase-3 is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis, procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and grazyme B. The active caspase-3 consists of large (17 kD) and small (11 kD) subunits. The active caspase-3 is involved in the proteolysis of poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with Alzheimer's disease. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (DEVD-AFC or DEVD-pNA) and is routinely tested for its ability to enzymatically cleave Ac-DEVD-pNA or Ac-DEVD-AFC. Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. Recommend using 1 unit/assay for analyzing caspase activity.

Biological Activity

Specific activity ≥ 15000 units/mg.

Quality Control

Quality Control & DataSheet

View current batch:
    Purity:N/A.
    Endotoxin Level:N/A
 

Description

Gene ID: 836 Accession #: P42574
Alternate Names:Active Human Caspase-3, Active Caspase-3, Caspase-3 Enzyme, Recombinant caspase-3, human caspase-3, human caspases, active caspases, CPP32, Yama, Apopain.
Source: Escherichia Coli. M.Wt: large (17 kD) and small (11 kD) subunits
Appearance: Semi-Dry powder Storage: Store at -70℃
Formulation:Semi-Dry powder
Solubility:Reconstitute in PBS containing 15% glycerol.
Shipping Condition: Gel pack. Handling: Centrifuge the vial prior to opening.
Usage:For Research Use Only! Not to be used in humans.