Caspase-3, human recombinant proteinase
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspase-3 is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis, procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6, caspase-8 and grazyme B. The active caspase-3 consists of large (17 kD) and small (11 kD) subunits. The active caspase-3 is involved in the proteolysis of poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. Caspase-3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with Alzheimer's disease. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (DEVD-AFC or DEVD-pNA) and is routinely tested for its ability to enzymatically cleave Ac-DEVD-pNA or Ac-DEVD-AFC. Active caspase-3 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. Recommend using 1 unit/assay for analyzing caspase activity.
|Specific activity||≥ 15000 units/mg.|
Quality Control & DataSheet
- View current batch:
|Gene ID:||836||Accession #:||P42574|
|Alternate Names:||Active Human Caspase-3, Active Caspase-3, Caspase-3 Enzyme, Recombinant caspase-3, human caspase-3, human caspases, active caspases, CPP32, Yama, Apopain.|
|Source:||Escherichia Coli.||M.Wt:||large (17 kD) and small (11 kD) subunits|
|Appearance:||Semi-Dry powder||Storage:||Store at -70℃|
|Solubility:||Reconstitute in PBS containing 15% glycerol.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|