VEGF165, human recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Vascular Endothelial Growth Factor (VEGF), also known as vascular permeability factor, is a potent growth and angiogenic cytokine produced in vascularized tissues. VEGF is a potent mediator of both angiogenesis and vasculogenesis, and stimulates proliferation and survival of endothelial cells. VEGF signals through the three receptors: fms-like tyrosine kinase (flt-1), KDR and flt4. VEGF induces angiogenesis, vasculogenesis and endothelial cell growth, mediates increased vascular permeability, promotes cell migration, and inhibits apoptosis.
Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length. VEGF165 is the most abundant and potent isoform. Recombinant human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.
|Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-7.0 ng/ml.|
Quality Control & DataSheet
- View current batch:
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 ng/μg
Related Biological Data
|Gene ID:||7422||Accession #:||Q96KJ0|
|Alternate Names:||Vascular Endothelial Growth Factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen.|
|Source:||Escherichia Coli.||M.Wt:||38.2 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Sterile filtered and lyophilized with no additives.|
|Solubility:||Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at +4°C for 1 week and -20°C for future use.|
|Shipping Condition:||Blue ice.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|