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TNF-alpha, recombinant murine protein

Catalog No.
P1002
TNF-α, expressed in E. coli, suitable for cell culture.
Grouped product items
SizePriceStock Qty
10ug
$178.00
Ship with 5-10 days
50ug
$429.00
Ship with 5-10 days
1mg
$3,762.00
Ship with 5-10 days

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Background

Tumor Necrosis Factor-α (TNF-α) is a potent cytokine expressed as a transmembrane protein with 235 aa that is cleaved to the soluble monomer by TNF-α converting enzyme (TACE) and forms stable homotrimers. TNF-α is expressed in many cells and plays an important role in inflammation, viral replication, tumorigenesis, apoptosis, cell proliferation, differentiation, coagulation and lipid metabolism. TNF-α is mainly produced by macrophages when challenged with viruses or bacteria. TNF-α is cytotoxic for many tumor cells lines but induced activation, differentiation or proliferation in normal cells. Tumor necrosis factor receptor TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR are receptors for murine TNF-α.

Biological Activity

Determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
ED50 < 0.1 ng/ml.          
Specific activity > 1*107 units/mg.          

Quality Control

Quality Control & DataSheet

View current batch:
    Purity: ≥98%, by SDS-PAGE and HPLC.
    Endotoxin Level:<0.1 ng/μg

Related Biological Data

TNF-alpha

Related Biological Data

TNF-alpha

Related Biological Data

TNF-alpha
 

Description

Gene ID: 21926 Accession #: P06804.2
Alternate Names:TNF alpha; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha.
Source: Escherichia Coli. M.Wt: 17.4 kDa
Appearance: Lyophilized powder Storage: Store at -20°C
Formulation:Steriled and lyophilized from 5 mM Tris, pH 8.0.
Solubility:Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.
Shipping Condition: Gel pack. Handling: Centrifuge the vial prior to opening.
Usage:For Research Use Only! Not to be used in humans.