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Scrambled 10Panx
Panx-1 mimetic inhibitory peptide, blocks pannexin-1 gap junctions

Scrambled 10Panx

Catalog No. A2701
Size Price Stock Qty
1mg $50.00 In stock
5mg $100.00 In stock
10mg $180.00 In stock
25mg $400.00 In stock

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Sample solution is provided at 25 µL, 10mM.

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Quality Control & MSDS

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Chemical structure

Scrambled 10Panx

Biological Activity

Description Scrambled 10Panx, Panx-1 mimetic inhibitory peptide, is a blocker of pannexin-1 gap junctions.
Targets Gap Channels          
IC50            

Protocol

Cell experiment [1]:

Cell lines

Cultured human subcutaneous fibroblasts.

Preparation method

Soluble to 0.50 mg/ml in sterile water. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

100 μM.

Applications

In cultured human subcutaneous fibroblasts, 10panx decreases the bradykinin-induced [Ca2+]i response and attenuates bradykinin (30 μM)-induced ATP release [1]. In oocytes, 10panx inhibits pannexin1 currents. In oocytes expressing pannexin1, 10panx attenuates dye uptake by pannexin1 channels [2].

References:

[1]. Pinheiro AR, Paramos-de-Carvalho D, Certal M, et al. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Cell Commun Signal, 2013, 11: 70.

[2]. Wang J, Ma M, Locovei S, et al. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol. 2007, 293(3): C1112-1119.

Scrambled 10Panx Dilution Calculator

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Chemical Properties

Cas No. 1315378-72-3 SDF Download SDF
Synonyms Scrambled 10Panx Phe-Ser-Val-Tyr-Trp-Ala-Gln-Ala-Asp-Arg
Canonical SMILES CC(C)C(C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CC2=CNC3=CC=CC=C32)C(=O)NC(C)C(=O)NC(CCC(=O)N)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CO)NC(=O)C(CC4=CC=CC=C4)N
Formula C58H79N15O16 M.Wt 1242.37
Solubility Soluble to 0.50 mg/ml in sterile water Storage Store at -20°C
General tips No
Shipping Condition No

Background

Scrambled 10Panx is the scrambled form of 10Panx (WRQAAFVDSY), a mimetic peptide of pannexin 1 that inhibits dye uptake by macrophages without affecting cellular membrane currents. Being structurally similar to anther peptide PanxE1B, 10Panx is three amino acid shorter but exerting an inhibitory effect against pannexin1 currents in oocytes to a similar extent as PanxE1b. 10Panx is also able to block the activation of I2nd, decrease the bradykinin-induced [Ca2+]i response and attenuate bradykinin-induced ATP release from human fibroblasts loaded with quinacrine. However, in several studies, scrambled 10Panx has been proved to be less effective than 10Panx in its authentic form.

Reference

Roger J. Thompson, Michael F. Jackson, Michelle E. Olah, Ravi L. Rungta, Dustin J. Hines, Michael A. Beazely, John F. MacDonald, Brian A. MacVicar. Activation of Pannexin-1 hemichannels augments aberrant bursting in the hippocampus. Science 2008; 322: 1555-1559.

Ana Rita Pinheiro, Diogo Paramos-de-Carvalho, Marianna Certal, Cristina Costa, Maria Teresa Magalhaes-Cardoso, Fatima Ferreirinha, Maria Adelina Costa and Paulo Correia-de-Sa. Bradykinin-induced Ca2+ signaling in human subcutaneous fibroblasts involves ATP release via hemichannels leading to P2Y12 receptors activation. Communication and Signaling 2013, 11:70

Junjie Wang, Meiyun Ma, Silviu Locovei, Robert W. Keane and Gerhard Dahl. Modulation of membrane channel currents by gap junction protein mimetic peptides: size matters. Am J Physiol Cell Physiol 293: C1112-C1119, 2007