In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 1 μM (binds to NGF) 
Ro 08-2750 is an antagonist of nerve growth factor (NGF), which selectively inhibits binding to p75NTR over TRKA147. This selectivity was used to study the functions of p75NTR and TrkA in apoptosis, neuronal differentiation and neurite outgrowth. NGF is a neuropeptide mainly involved in the regulation of growth, proliferation, maintenance, and survival of certain target neurons.
In vitro: Ro 08-2750 can bind to the NGF dimer with ~ 1:1 binding ratio. Binding of Ro 08-2750 to NGF induces NGF a concentration-dependent conformational change . Binding with low concentration of Ro 08-2750, NGF can no longer bind to p75NTR anymore, however, binding to TrkA is not impaired. At higher concentrations of Ro 08-2750 more than 5 mM, the NGF/Ro 08-2750 complex can not bind to TrkA as well. In SK-N-MC cells, Ro 08-2750 leads to inhibition of NGF-induced programmed cell death. In PC12 cells, Ro 08-2750 can enhance the signal pathway through TrkA.
In vivo:. So far, in vivo study has been conducted.
Clinical trial: So far, no clinical study has been conducted.
 Arkin MR, Wells JA. Small-molecule inhibitors of protein-protein interactions: progressing towards the dream. Nat Rev Drug Discov. 2004 Apr;3(4):301-17.
 Niederhauser O, Mangold M, Schubenel R, Kusznir EA, Schmidt D, Hertel C. NGF ligand alters NGF signaling via p75(NTR) and trkA. J Neurosci Res. 2000 Aug 1;61(3):263-72.
|Physical Appearance||Orange solid|
|Storage||Store at -20°C|
|Solubility||≤2.7mg/ml in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|