In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PSI-6206 (as known as RO-2433 or GS-331007), β-D-2’-deoxy-2’-fluoro-2’-C-methyluridine, is the deaminated derivative of PSI-6130, β-D-2’-deoxy-2’-fluoro-2’-C-methylcytidine, which is a potent inhibitor of hepatitis C virus (HCV) replication in the subgenomic HCV reolicon system. PSI-6206, itself, does not shown any inhibitory activity towards HCV replication in the HCV subgenomic replicon system. However, the its triphosphate form, RO2433-TP, is a potent inhibitor of RNA synthesis by HCV polymerase, which inhibits both the RNA sysnthesis activity of HCV replicase (IC50 = 1.19 uM) and the RNA synthesis activity of the recombinant HCV Con1 NS58 on a heteropolymeric RNA template derived from the 3’-end of the negative strand of the HCV genome (IC50 = 0.52 uM and Ki = 0.141 uM).
Han Ma, Wen-Rong Jiang, Nicole Robledo, Vincent Leveque, Samir Ali, Teresa Lara-Jamie, Mohammad Masjedizadeh, David B. Smith, Nick Cammack, Klaus Klumpp, and Julian Symons. Characterization of the metabolic ctivation of hepatitis C virus nucleoside inhibitor β-D-2’-deoxy-2’-fluoro-2’-C-methylcytidine (PSI-6130) and identification of a novel active 5’-triphosphate species. The Journal of Biological Chemistry 2007; 282(41): 29812-29820
- 1. Eyer L, Šmídková M, et al. "Structure-activity relationships of nucleoside analogues for inhibition of tick-borne encephalitis virus. Antiviral Res." 2016 Jul 28;133:119-129. PMID:27476046
- 2. Eyer L, Nencka R, et al. "Nucleoside inhibitors of Zika virus." J Infect Dis. 2016 May 27. pii:jiw226. PMID:27234417
- 3. Rower, Joseph E, et al. "Validation and application of an LC-MS/MS method to determine the concentrations of sofosbuvir anabolites in cells." Antimicrobial agents and chemotherapy (2015): AAC-01693. PMID:26416874
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥13.1mg/mL in DMSO, ≥24.15 mg/mL in EtOH with ultrasonic, ≥48.7 mg/mL in H2O with ultrasonic|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Description||PSI-6206 (RO2433) is a selective inhibitor of HCV RNA polymerase .|
Quality Control & MSDS
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