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Prednisone

Catalog No.
B2148
Glucocorticoid receptor agonist
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$80.00
In stock
1g
$57.00
In stock
5g
$143.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Prednisone (Adasone) is a synthetic corticosteroid agent that is particularly effective as an immunosuppressant compound.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt358.43
Cas No.53-03-2
FormulaC21H26O5
Solubilityinsoluble in H2O; insoluble in EtOH; ≥15.35 mg/mL in DMSO
Chemical Name(8S,9S,10R,13S,14S,17R)-17-hydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-6,7,8,9,12,14,15,16-octahydrocyclopenta[a]phenanthrene-3,11-dione
SDFDownload SDF
Canonical SMILESCC12CC(=O)C3C(C1CCC2(C(=O)CO)O)CCC4=CC(=O)C=CC34C
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

Peripheral blood lymphocytes (PBL) or peripheral CD4+ and CD8+ T cell subsets

Preparation method

The solubility of this compound in DMSO is > 15.4 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

10-3 ~ 10-12 M; 72, 96 and 120 hrs

Applications

Prednisone arrested PBL in the G1 phase of cell cycle, and inhibited IL-2 receptor (IL-2R) expression as well as IL-2 secretion. In phytohaemaglutinin (PHA)-activated human PBL, Prednisone significantly increased apoptosis. Moreover, Prednisone exhibited stronger apoptotic effect on CD8+ T lymphocytes than on CD4+ T lymphocytes. All these effects were shown in dose- and time-dependent manners.

Animal experiment [2]:

Animal models

Male Wistar rats

Dosage form

5 mg/kg/d; p.o.; for 90 days

Applications

In Wistar rats, Prednisone significantly delayed learning and memory retention by 20%. Compared with the control group, the neuronal degeneration index in the Prednisone treatment group was 2 times higher in the prefrontal cortex, and about 10 times higher in the CA1 hippocampus. In addition, Prednisone substantially increased the number and cytoplasmic transformation of astrocytes, as well as isolectin-B4-labeled microglia cells in the prefrontal cortex.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Lanza L, Scudeletti M, Puppo F, Bosco O, Peirano L, Filaci G, Fecarotta E, Vidali G, Indiveri F. Prednisone increases apoptosis in in vitro activated human peripheral blood T lymphocytes. Clin Exp Immunol. 1996 Mar;103(3):482-90.

[2]. Ramos-Remus C, González-Castaeda RE, González-Perez O, Luquin S, García-Estrada J. Prednisone induces cognitive dysfunction, neuronal degeneration, and reactive gliosis in rats. J Investig Med. 2002 Nov;50(6):458-64.

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