PDGF-BB, murine recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The platelet-derived growth factor BB (PDGF-BB) is a member of the PDGF family and is a disulfide-linked homodimer. PDGF family consists of proteins PDGF-A, -B that form three disulfide-linked homodimers (PDGF-AA, -BB and -AB). The PDGFs are stored in platelet α-granules, and are released upon platelet activation. PDGFs signals through receptors PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. However, PDGFR-β interacts with only PDGF-BB and PDGF-AB. PDGF-BB is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells and some blood cells.
Recombinant murine PDGF-BB is a homodimeric, non-glycosylated polypeptide of 109 amino acids and having a molecular mass of 24.4 kDa.
|Determined by the dose-dependent proliferation of murine BALB/c 3T3 cells.|
|ED50||< 2 ng/ml.|
Quality Control & DataSheet
- View current batch:
Purity: ≥95%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 ng/μg/span>
Related Biological Data
|Gene ID:||18591||Accession #:||P31240|
|Alternate Names:||PDGFB, Murine PDGF-BB, m-PDGF-BB, PDGFBB, PDGF-BB, PDGF BB, rm-PDGF-BB, recombinant murine PDGF-BB, recombinant PDGF-BB, PDGF.|
|Source:||Escherichia Coli.||M.Wt:||24.4 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Lyophilized with no additives.|
|Solubility:||Reconstitute in sterile 100mM acetic acid and 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|