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Nitrocefin

Catalog No.
B6052
Used for detection of β-lactamases
Grouped product items
SizePriceStock Qty
1mg
$45.00
In stock
5mg
$75.00
In stock
10mg
$140.00
In stock
25mg
$325.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Nitrocefin is a chromogenic cephalosporin substrate which is used for detection of β-lactamases. β-lactamases are enzymes produced by various microbes that mediate resistance to β-lactam antibiotics such as penicillins and  cephamycins. Comparing to other methods for β-lactamases detection, nitrocefin is more sensitive while using few materials and inexpensive equipment. The color change by nitrocefin can be followed quantitatively by measuring changes in absorption between 380 to 500 nm region[1].

Reference:
[1] O'Callaghan, Cynthia H et al.  Novel Method for Detection of B-Lactamases by Using a Chromogenic Cephalosporin Substrate. Antimicrobial Agents and Chemotherapy. 1972 Apr; 1(4):283-288.

Product Citation

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt516.50
Cas No.41906-86-9
FormulaC21H16N4O8S2
Solubilityinsoluble in EtOH; insoluble in H2O; ≥20.24 mg/mL in DMSO
Chemical Name(6R,7R)-3-((E)-2,4-dinitrostyryl)-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
SDFDownload SDF
Canonical SMILES[H][[email protected]]1([[email protected]@H]2NC(CC3=CC=CS3)=O)SCC(/C=C/C4=CC=C([N+]([O-])=O)C=C4[N+]([O-])=O)=C(C(O)=O)N1C2=O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control