Toggle Nav
Close
  • Menu
  • Setting

NHS-12-Biotin

Catalog No.
A8784
Amine-reactive biotinylation reagent, long chain
Grouped product items
SizePriceStock Qty
100mg
$150.00
In stock
1g
$800.00
Ship with 10-15 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

NHS-12-Biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end. The NHS ester group at the end of NHS-12-Biotin covalently binds to amine groups in proteins and other molecules forming a stable amide linkage and releasing the NHS group. NHS-12-Biotin has a greater length between a covalently modified molecule and the bicyclic biotin rings than NHS-LC-Biotin, which provides the avidin or streptavidin probes a greater opportunity to reach the binding sites deeply buried inside the surface plane of proteins. NHS-12-Biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction.

Chemical Properties

StorageStore at -20°C
M.Wt539.64
FormulaC24H37N5O7S
Solubility≥20.65 mg/mL in EtOH; ≥23.15 mg/mL in H2O; ≥44.5 mg/mL in DMSO
Chemical Name2,5-dioxopyrrolidin-1-yl 4-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)butanoate
SDFDownload SDF
Canonical SMILESO=C1N[[email protected]@]([H])([[email protected]]([H])(CS2)N1)[[email protected]@H]2CCCCC(NCCCCCC(NCCCC(ON3C(CCC3=O)=O)=O)=O)=O
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control