|Hexa His tag peptide Synthetic 6XHis peptide|
Sample solution is provided at 25 µL, 10mM.
2. Bartoschik T, Galinec S, et al. "Near-native,site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis." Sci Rep. 2018 Mar 21;8(1):4977. PMID:29563556
3. Guo M, Tang X, et al. "The Immune Adjuvant Effects of Flounder (Paralichthys olivaceus) Interleukin-6 on E.tarda Subunit Vaccine OmpV." Int J Mol Sci. 2017 Jul 5;18(7). pii: E1445. PMID:28678171
|Description||Hexa His tag peptide is a 6 x His tag used as a metal binding site for the recombinant protein.|
|Targets||anti-6 x His tag antibody|
|Cas No.||N/A||SDF||Download SDF|
|Chemical Name||Hexa His|
|Solubility||>84.1mg/ml in DMSO or water||Storage||Desiccate at -20°C|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
Hexa His is an amino acid sequence consisting of 6 His residues in a row, it is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Hexa His Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. Hexa His acts as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.
1. Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285–6.