Recombinant Flagellin, His
Catalog No.
P1644
Featured Products
Flagellin arranges itself in a hollow cylinder to form the filament in bacterial flagellum. It is the principal substituent of bacterial flagellum, and is present in large amounts on nearly all flagellated bacteria. Mammals often have acquired immune responses (T-cell and antibody responses) to flagellated bacterium and the plant defense mechanisms can be activated by the conserved N-terminal part of flagellin.
Reference
1. Fedorov OVandKostyukova AS. 1984. FEBS Lett, 171: 145-8
2. Belas RandFlaherty D. 1994. Gene, 148: 33-41
3. Givaudan A, Lanois A, Boemare N. 1996. Gene, 183: 243-53
4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12: 1783-94
5. Hayashi F, Smith KD, Ozinsky A, et al. 2001. Nature, 410: 1099-103.
| Gene ID | 1253480 |
| Accession # | P06179 |
| Source | Escherichia coli. |
| M.Wt | Approximately 52.7 kDa, a single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus. |
| AA Sequence | MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD L |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Data is not available. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 95 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/µg of rFlagellin, His as determined by LAL method.