HBC
Catalog No.
A8786
For the production of fluorescent RNAs, used in live imaging of cellular RNAs.
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HBC (HBC 530) is a synthetic dye similar to GFP fluorophores, featuring a structurally rigid electron acceptor and strong electron donor structure. HBC itself does not fluoresce in solution, but when it binds to Pepper (RNA aptamer), it forms a tight complex, activates and emits bright fluorescence, with a binding constant (Kd) of approximately 3.5 nM. The emission peaks of HBC vary in different Pepper complexes, covering a visible spectrum ranging from cyan to red. The typical excitation/emission wavelength of HBC 530 is 485/530 nm, which is highly suitable for biological imaging applications such as live cell imaging of RNA.
| Storage | Store at -20°C away from light for 3 years. |
| M.Wt | 303.36 |
| Cas No. | 156840-13-0 |
| Formula | C19H17N3O |
| Solubility | insoluble in EtOH; insoluble in H2O; ≥21.2 mg/mL in DMSO |
| Chemical Name | (E)-4-(1-cyano-2-(4-((2-hydroxyethyl)(methyl)amino)phenyl)vinyl)benzonitrile |
| SDF | Download SDF |
| Canonical SMILES | OCCN(C)C1=CC=C(/C=C(C#N)\C2=CC=C(C#N)C=C2)C=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure
