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Cy5 NHS ester (non-sulfonated)

Catalog No.
Labeling of amino-groups in biomolecules.
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
In stock
In stock

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Cyanine5 NHS ester is a ideal reactive dye for the labeling of peptides, proteins, and oligonucleotides which contain the amino-groups. Recently, among the life science research and diagnostics, Dye color quantity can be detected in gel electrophoresis by naked eye as the dye color is very intense and as small as 1 nanomol. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble Cy5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.

In mice, Cy5-labeled polyplexes was intravenous injected to evaluate the targeting ability of polyplexes in tumor-bearing nude mice. Heart, liver, spleen, lung, kidney and tumor, were removed after the mice were sacrificed and the fluorescence intensities of Cy5-labeled polyplexes were immediately examined [1].

[1] Chen, K. ; Chen, Q.; Wang, K.; Zhu, J.; Li, W.; Li, W.; Qiu, L.; Guan, G.; Qiao, M.; Zhao, X.; Hu, H.; Chen, D. Synthesis and characterization of a PAMAM-OH derivative containing an acid-labile β-thiopropionate bond for gene delivery. International Journal of Pharmaceutics, 2016, 509(1–2), 314–327.

Product Citation

Chemical Properties

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Solubility≥30.8mg/mL in DMSO, ≥15.1 mg/mL in EtOH with ultrasonic,insoluble in H2O
Chemical Name(2,5-dioxopyrrolidin-1-yl) 6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanoate
SDFDownload SDF
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Spectral properties

Excitation max (nm) 646 Emission max (nm) 662
Extinction Coefficient (M-1cm-1) 250000 Quantum Yield 0.2
CF260 0.03 CF280 0.04

Quality Control

Chemical structure

Cy5 NHS ester (non-sulfonated)

Related Biological Data

Cy5 NHS ester (non-sulfonated)

Related Biological Data

Cy5 NHS ester (non-sulfonated)