Cy3 NHS ester (non-sulfonated)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cyanine, a kind of synthetic dye belonging to the polymethine group, is still used in industry to increase the sensitivity range of photographic emulsions (such as increasing the wavelength range and making the film panchromatic), and in biotechnology more recently. As fluorescent dyes, cyanines have been particularly used in biomedical imaging. Depending on their structure, cyanines can cover the spectrum from UV to IR.
Cy3 NHS ester, a reactive dye for labeling of amino-groups in biomolecules, is a Cy3® NHS ester analog. This reagent is commonly used for the labeling of soluble proteins, peptides, and oligonucleotides or DNA. Cy3 NHS ester requires the use of organic co-solvent (such as DMF, or DMSO) for the labeling, however, considering the usage of water-soluble sulfo-Cy3 NHS ester for delicate proteins, such co-solvent is not necessary. Cy3 fluoresce is in the orange region (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the red (~650/670 nm) but absorbs in the orange (~649 nm). Cy3 can be monitored by fluorometers, imagers, and microscopes with standard Tetramethylrhodamine (TRITC) filters. Due to its inherently high extinction coefficient, Cy3 is even detected by naked eye on gels and in solution easily.
|Physical Appearance||A solid|
|Storage||24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.|
|Solubility||≥59mg/mL in DMSO, ≥25.3 mg/mL in EtOH with ultrasonic,insoluble in H2O|
|Chemical Name||(2,5-dioxopyrrolidin-1-yl) 6-[(2E)-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethylindol-1-ium-2-yl)prop-2-enylidene]indol-1-yl]hexanoate|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Excitation max (nm)||555||Emission max (nm)||570|
|Extinction Coefficient (M-1cm-1)||150000||Quantum Yield||0.31|
Quality Control & MSDS
- View current batch:
Purity = 98.00%
- COA (Certificate Of Analysis)
- MS (Mass Spectrometry) (Retest)
- MSDS (Material Safety Data Sheet)