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Caffeine

Catalog No.
N2379
 
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$60.00
In stock
20mg
$60.00
In stock

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Background

Extracted from tea, coffee fruit; Store the product in sealed, cool and dry condition

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt194.19
Cas No.58-08-2
FormulaC8H10N4O2
Solubility≥25 mg/mL in H2O
Chemical Name1,3,7-trimethylpurine-2,6-dione
SDFDownload SDF
Canonical SMILESCN1C=NC2=C1C(=O)N(C(=O)N2C)C
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1]:

Cell lines

Patient-derived AC-UPS01 and AC-RMS01 cell lines

Preparation method

The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Reacting condition

0.25, 0.5, 1, 2.5 and 5 mM; 72 h

Applications

Caffeine significantly inhibited the patient-derived UPS and RMS cell lines in a dose-dependent manner with IC50 values of 2.02 ± 0.22 mM and 2.37 ± 0.48 mM, respectively. Addition of 0.3 mM or 0.6 mM VPA to varying concentrations of CAF enhanced efficacy against both the AC-UPS01 and AC-RMS01 cell lines.

Animal experiment [2]:

Animal models

diet-induced obesity (DIO) mice

Dosage form

10 μg per mouse, i.c.v. administration

Application

In diet-induced obesity (DIO) mice, administration of caffeine into mouse brain significantly increased the numbers of c-Fos+ cells in the PVN, Arc and DMH nuclei, suggesting that caffeine stimulated the activities of neurons in the hypothalamic nuclei involved in energy balance control. Caffeine reduced the adipocyte sizes of epididymal white adipose tissue, plasma triglycerides (TG) levels and improved glucose tolerance. Mice gained significantly less body weights than the controls on day 7.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1] Igarashi K1,2,3, Kawaguchi K1,2, Kiyuna T1,2, et al. Efficacy In Vitro of Caffeine and Valproic Acid on Patient-Derived Undifferentiated Pleomorphic Sarcoma and Rhabdomyosarcoma Cell Lines. Anticancer Res. 2017 Aug; 37 (8): 4081-4084.

[2] Wu L1, Meng J1, Shen Q1, et al. Caffeine inhibits hypothalamic A1R to excite oxytocin neuron and ameliorate dietary obesity in mice. Nat Commun. 2017 Jun 27; 8: 15904.

Quality Control

Chemical structure

Caffeine

Related Biological Data

Caffeine