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GenuinePrep Tn5 Transposase

Catalog No.
K1155
GenuinePrep Tn5 Transposase efficiently inserts Tn5 transposon into target sequences
Grouped product items
SizePriceStock Qty
20ul
$360.00
In stock
100ul
$1,620.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

GenuinePrep Tn5 Transposase is a highly active mutant of wild type Tn5 Transposase and can efficiently insert Tn5 transposon into a target DNA sequence in vitro. The GenuinePrep Tn5 Transposase specifically recognizes the mosaic end (ME) sequences of inverted repeats at both ends of Tn5 transposon. GenuinePrep Tn5 Transposase generate transposition efficiency 1000-fold higher in vitro than the wild type.

The insertion site of GenuinePrep Tn5 Transposase has high randomness, and double-stranded DNA fragments containing paired recognition sequences can be randomly integrated into the genome of the host cells (in vitro transgene manipulation). In addition, GenuinePrep Tn5 Transposase often used in Next-Generation Sequencing (NGS) and other fields.

Quality Control

Quality Control & MSDS

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Components and Storage

Components 20 μL 100 μL
GenuinePrep Tn5 Transposase (400 ng/μL)* 20 μL 100 μL
Annealing Buffer 400 μL 1 mL x 2
Coupling Buffer 200 μL 1 mL
5X Reaction Buffer 200 μL 1 mL

Store the GenuinePrep Tn5 Transposase at -80°C, and store other components at -20°C.

The prepared transposome should be stored at -20°C and is stable for at least one year.

*Note: The mass concentration of GenuinePrep Tn5 Transposase is 400 ng/μL, which is converted to a molar concentration of 7.5 pmol/μL.

Features

1. One tube enzymatic reaction, easy to operate

2. High DNA fragmentation activity

3. Each component provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.

Storage

Store the GenuinePrep Tn5 Transposase at -80°C, and store other components at -20°C. The prepared transposome should be stored at -20°C and is stable for at least one year.