Caspase Fluorometric Substrate Set Plus
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Caspases (Cysteine-dependent aspartate-directed proteases) are a family of cysteine proteases that play important roles in apoptosis, inflammation and necrosis. Sequential activation of caspases plays a critical role in cell apoptosis. Caspase-1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 are members of caspases family. Caspase-1, 4 and 5 are involved in T-cell maturation. Caspase-2, 8, 9 and 10 are initiator caspases that cleave inactive pro-forms of effector caspases into active caspases. Caspase-3, 6 and 7 are effector caspases that cleave other protein substrates within the cell to trigger the apoptotic process.
Caspase Fluorometric Substrate Set Plus is composed of ready-to-use AFC-labeled substrates for caspase-1, 2, 3, 5, 6, 8 and 9 of caspase family proteases. The kit is used to detect activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
| Features | Contains ready-to-use caspase-1/-2/-3/-5/-6/-8/-9 AFC-labeled substrates and all buffers for performing caspase assay. |
| Shipping | Gel pack. |
| Storage Conditions | Store at -20°C. |
| Kit components | Caspase-1 Substrate, Ac-YVAD-AFC, Caspase-2 Substrate, Ac-VDVAD-AFC, Caspase-3 Substrate, Ac-DEVD-AFC, Caspase-5 Substrate, Ac-WEHD-AFC, Caspase-6 Substrate, Ac-VEID-AFC, Caspase-8 Substrate, Ac-IETD-AFC, Caspase-9 Substrate, Ac-LEHD-AFC, Cell Lysis Buffer, 2X Reaction Buffer, DTT (1 M) |