Home
U 73343

U 73343

Catalog No.

B9013

A negative control of the putative phospholipase C inhibitor U 73122

Skip to the beginning of the images gallery

Grouped product items
Size	Price	Stock
5mg	$139.00	Ship with 5-10 days
10mg	$231.00	Ship with 5-10 days
25mg	$519.00	Ship with 5-10 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background
Chemical Properties
Protocol
Quality Control

Background

U 73343 is an inactive analog of the putative phospholipase C antagonist U 73122. Phospholipase C is a class of membrane-associated enzymes, which plays an important role in signal transduction pathways by cleaving phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate, which serve as second messengers. Therefore, U 73343 can be used as a negative control of U 73122 for elucidating the role of phospholipase C in a variety of biological events such as gastric acid secretion and hormone-stimulated calcium signals.

References:

1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388.

2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582.

Chemical Properties

Physical Appearance	A crystalline solid
Storage	Store at -20°C
M.Wt	466.66
Cas No.	142878-12-4
Formula	C₂₉H₄₂N₂O₃
Solubility	Soluble in DMSO
Chemical Name	1-(6-(((8R,9S,13S,14S,17S)-3-methoxy-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthren-17-yl)amino)hexyl)pyrrolidine-2,5-dione
SDF	Download SDF
Canonical SMILES	C[C@]12CC[C@]3([H])C4=CC=C(OC)C=C4CC[C@@]3([H])[C@]1([H])CC[C@]2([H])NCCCCCCN5C(CCC5=O)=O
Shipping Condition	Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips	We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[2]
Cell lines	UMR-106 osteoblastic cells
Reaction Conditions	6 ~ 8 μM U 73343 for 3 min incubation
Applications	At the concentration range of 6 ~ 8 μM, U 73343 elicited no effects on endothelin-1 and parathyroid hormone stimulated intracellular calcium transients, whereas U 73122 exhibited complete inhibition on responses to 100 nM endothelin-1 or parathyroid hormone. Also, U 73343 did not affect endothelin-1-induced inositol trisphosphate production.
Note	The technical data provided above is for reference only.
	References: 1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388. 2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582.

Quality Control

Quality Control & MSDS

View current batch:

Purity ≥ 95.00%

Chemical structure

U 73343

mRNA synthesis

Tyramide Signal Amplification (TSA)

Phos Binding Reagent Acrylamide

Cell Counting Kit-8 (CCK-8)

Inhibitor Cocktails

Background

Chemical Properties

Protocol

Quality Control

Quality Control & MSDS

Chemical structure

Product Catalog

Order Info

Customer Service

Contact Us

Newsletter

Follow Us