U 73343
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
U 73343 is an inactive analog of the putative phospholipase C antagonist U 73122. Phospholipase C is a class of membrane-associated enzymes, which plays an important role in signal transduction pathways by cleaving phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate, which serve as second messengers. Therefore, U 73343 can be used as a negative control of U 73122 for elucidating the role of phospholipase C in a variety of biological events such as gastric acid secretion and hormone-stimulated calcium signals.
References:
1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388.
2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582.
| Physical Appearance | A crystalline solid |
| Storage | Store at -20°C |
| M.Wt | 466.66 |
| Cas No. | 142878-12-4 |
| Formula | C29H42N2O3 |
| Solubility | Soluble in DMSO |
| Chemical Name | 1-(6-(((8R,9S,13S,14S,17S)-3-methoxy-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthren-17-yl)amino)hexyl)pyrrolidine-2,5-dione |
| SDF | Download SDF |
| Canonical SMILES | C[[email protected]]12CC[[email protected]]3([H])C4=CC=C(OC)C=C4CC[[email protected]@]3([H])[[email protected]]1([H])CC[[email protected]]2([H])NCCCCCCN5C(CCC5=O)=O |
| Shipping Condition | Ship with blue ice, or upon other requests. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[2] | |
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Cell lines |
UMR-106 osteoblastic cells |
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Reaction Conditions |
6 ~ 8 μM U 73343 for 3 min incubation |
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Applications |
At the concentration range of 6 ~ 8 μM, U 73343 elicited no effects on endothelin-1 and parathyroid hormone stimulated intracellular calcium transients, whereas U 73122 exhibited complete inhibition on responses to 100 nM endothelin-1 or parathyroid hormone. Also, U 73343 did not affect endothelin-1-induced inositol trisphosphate production. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388. 2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582. |
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Quality Control & MSDS
- View current batch:
-
Purity ≥ 95.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
