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U 73343

Catalog No.
B9013
A negative control of the putative phospholipase C inhibitor U 73122
Grouped product items
SizePriceStock Qty
5mg
$104.00
Ship with 5-10 days
10mg
$173.00
Ship with 5-10 days
25mg
$390.00
Ship with 5-10 days

Tel: +1-832-696-8203

Email: [email protected]

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Background

U 73343 is an inactive analog of the putative phospholipase C antagonist U 73122. Phospholipase C is a class of membrane-associated enzymes, which plays an important role in signal transduction pathways by cleaving phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate, which serve as second messengers. Therefore, U 73343 can be used as a negative control of U 73122 for elucidating the role of phospholipase C in a variety of biological events such as gastric acid secretion and hormone-stimulated calcium signals. 

References:

1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388.

2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582.

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt466.66
Cas No.142878-12-4
FormulaC29H42N2O3
SolubilitySoluble in DMSO
Chemical Name1-(6-(((8R,9S,13S,14S,17S)-3-methoxy-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthren-17-yl)amino)hexyl)pyrrolidine-2,5-dione
SDFDownload SDF
Canonical SMILESC[[email protected]]12CC[[email protected]]3([H])C4=CC=C(OC)C=C4CC[[email protected]@]3([H])[[email protected]]1([H])CC[[email protected]]2([H])NCCCCCCN5C(CCC5=O)=O
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[2]

Cell lines

UMR-106 osteoblastic cells

Reaction Conditions

6 ~ 8 μM U 73343 for 3 min incubation

Applications

At the concentration range of 6 ~ 8 μM, U 73343 elicited no effects on endothelin-1 and parathyroid hormone stimulated intracellular calcium transients, whereas U 73122 exhibited complete inhibition on responses to 100 nM endothelin-1 or parathyroid hormone. Also, U 73343 did not affect endothelin-1-induced inositol trisphosphate production.

Note

The technical data provided above is for reference only.

References:

1. Muto Y, Nagao T, Urushidani T. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. Journal of Pharmacology and Experimental Therapeutics, 1997, 282(3): 1379-1388.

2. Tatrai A, Lee SK, Stern PH. U-73122, a phospholipase C antagonist, inhibits effects of endothelin-1 and parathyroid hormone on signal transduction in UMR-106 osteoblastic cells. Biochimica et Biophysica Acta, 1994, 1224(3): 575-582.

Quality Control

Quality Control & MSDS

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Chemical structure

U 73343