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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
Rabbit pulmonary artery myocytes
Preparation method
The solubility of this compound in DMSO is >10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
Whole cell IClCa were evoked by pipette solution which had the following composition (in mM): TEA (20), CsCl (106), HEPES-CsOH (10, pH 7.2), BAPTA (10), ATP.Mg (5) and GTP.diNa (0.2). To this solution, 7.08 mM CaCl2 were added to achieve a free [Ca2+] of 500 nM. The bathing solution had the following composition (in mM): NaCl (126), HEPES-NaOH (10, pH 7.35), TEA (8.4), glucose (20), MgCl2 (1.2) and CaCl2 (1.8). The effect of T16Ainh-A01 (1–30 μM) was studied on IClCa evoked by 500 nM free Ca2+.
Applications
In rabbit pulmonary artery myocytes T16Ainh-A01 (1–30 μM) inhibited single calcium (Ca2+)-activated whole cell currents activated by 500 nM free Ca2+.
References:
[1] Alison J Davis, et al. Potent vasorelaxant activity of the TMEM16A inhibitor T16Ainh-A01. Br J Pharmacol. 2013 Feb; 168(3): 773–784.