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Catalog No.
Toll-like receptor 3 (TLR3) agonist
Grouped product items
SizePriceStock Qty
10mM (in 1mL H2O)
In stock
In stock
In stock

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Poly (I:C), a synthetic double-stranded RNA (dsRNA) analog, is an immunostimulant that acts as the most potent interferon (IFN) inducer. Toll-like receptor- 3 (TLR3) agonist[1].

Mixtures that included the TLR7/8 agonists R848 or CL075, combined with the Poly (I:C), yield 3-d mature dendritic cells [2].

Commonly used to stimulate immune system activation in vivo. Also promotes maturation of hPSC-derived cardiomyocytes[3]. 


[1]. Li Y G, Siripanyaphinyo U, Tumkosit U, et al. Poly (I:C), an agonist of toll-like receptor-3, inhibits replication of the Chikungunya virus in BEAS-2B cells. Virology Journal, 2012, 9(1): 114.

[2]. Spranger S, Javorovic M, Burdek M, et al. Generation of Th1-Polarizing Dendritic Cells Using the TLR7/8 Agonist CL075. The Journal of Immunology, 2010, 185(1): 738-747.

[3]. Biermann M1, Cai W2, Lang D, et al. Epigenetic Priming of Human Pluripotent Stem Cell-Derived Cardiac Progenitor Cells Accelerates Cardiomyocyte Maturation. Stem Cells, 2019, 37(7): 910-923.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
Cas No.24939-03-5
Solubility≥21.5mg/mL in H2O,insoluble in DMSO
Chemical Name((2R,3S,4R,5R)-3,4-dihydroxy-5-(6-oxo-3H-purin-9(6H)-yl)tetrahydrofuran-2-yl)methyl dihydrogen phosphate compound with ((2R,3S,4S,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate (1:1)
SDFDownload SDF
Canonical SMILESO[[email protected]@H]([[email protected]](N1C(NC=NC2=O)=C2N=C1)O[[email protected]@H]3COP(O)(O)=O)[[email protected]@H]3O.O[[email protected]]([[email protected]@H](COP(O)(O)=O)O[[email protected]]4N5C=CC(N)=NC5=O)[[email protected]@H]4O
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.


Cell experiment [1]:

Cell lines

Dendritic cell

Preparation method

Soluble in sterile water. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

12.5 mg/ml, 3 days


Treatment of immature DC for 72 h with poly(I:C) resulted in the typical DC morphology and induced significant maturation. Poly(I:C)-treated DC remained stable in phenotype after cytokine withdrawal. Poly(I:C)-treated DC down-regulated pinocytic activity. Poly(I:C)-treated DC produced high levels of IL-12 and low levels of IL-10.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.


[1]. Verdijk R M, Mutis T, Esendam B, et al. Polyriboinosinic polyribocytidylic acid (poly (I: C)) induces stable maturation of functionally active human dendritic cells[J]. The Journal of Immunology, 1999, 163(1): 57-61.

Quality Control

Chemical structure


Related Biological Data


Related Biological Data


Related Biological Data