Phosphate Colorimetric Assay Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
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Phos Binding Reagent Acrylamide
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Cell Counting Kit-8 (CCK-8)
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SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Phosphate is an important inorganic ion in biological systems and has many functions. Phosphate can turn on or off enzyme activity through the mediation of the various phosphatases and protein kinases in biological systems. Phosphate also plays an important role in mineralization processes and is a major stimulus of algal blooms mainly found in bodies of fresh water, because of run-off from areas of high fertilizer use.
The Phosphate Colorimetric Assay Kit provides a simple, fast and sensitive way for detection of phosphate over a wide range of concentrations in various samples based on colorimetric method. The assay uses a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion that can be easily detected at 650 nm using a spectrophotometer or an ELISA plate reader. The kit can dectet 1 µM - 1 mM phosphate concentrations with a lower limit of approximately 0.1 nmol. The kit provides 500 assays using microtiter plates or 100 assays using 1 mL cuvettes.
Quality Control & DataSheet
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|Kit components||Phosphate Reagent Phosphate Standard (10 mM)|
|Detection method||Absorbance (650 nm)|
|Sample type||Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids, algal blooms and water from run-off areas of high fertilizer use.|
Features & Properties
|Features||Fast and convenient Simple procedure; takes 30 minutes. The assay is sensitive, stable and high-throughput adaptable.|
|Storage Conditions||Room temperature.|