Neuregulin/Heregulin-1β (NRG-1β/HRG-1β), human recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Neuregulin (NRG) is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells and plays an important role in heart structure and function through inducing cardiomyocyte differentiation. Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). HRG1-β1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases ErbB3/ErbB4, which induces ErbB3 and ErbB4 heterodimerization with ErbB2 and leads to tyrosine phosphorylation. HRG1-β1 is involved in up-regulation of expression and function of the autocrine motility-promoting factor (AMF).
Recombinant human Heregulin-β1 (HRG1-β1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-β1 (65 amino acid residues).
|Determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells.|
|ED50||< 0.5 ng/ml.|
|Specific activity||> 2*106 units/mg.|
Quality Control & DataSheet
- View current batch:
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 ng/μg
Related Biological Data
|Gene ID:||3084||Accession #:||Q15491|
|Alternate Names:||NGR beta 1, Heregulin, HRG 1beta, Neuregulin, Neuregulin1 (NRG1-β1), Neu differentiation factor, Pro-neuregulin-1, Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, Glial growth factor, Heregulin, Neu differentiation factor, Sensory and motor neuron-derived factor.|
|Source:||Escherichia Coli.||M.Wt:||7.86 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Sterile Filtered lyophilized (freeze-dried) powder.|
|Solubility:||Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|