NADP/NADPH Quantitation Colorimetric Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
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Nicotinamide adenine dinucleotide phosphate (NADP+) is a cofactor in the lipid and nucleic acid biosynthesis, which require NADPH as a reducing agent. NADP+ plays important roles in energy transforming and redox state of cells or tissues.
The NADP/NADPH Quantitation Colorimetric Kit provides a sensitive and convenient way for detection of the intracellular nucleotides: NADP, NADPH and their ratio. The NADP Cycling Enzyme Mix specifically recognize NADP/NADPH in an enzyme cycling reaction, which are not required to purify from samples. The enzyme cycling reaction significantly increases the detection specificity and sensitivity. Results can be easily quantified using plate reader at OD450 nm. The assay specifically detects NADP and NADPH, but not NAD or NADH.
Features & Properties
|Features||Fast and convenient Simple procedure; takes ~2 hours. Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio. Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD+/NADH)|
|Storage Conditions||Store at -20°C.|
Quality Control & DataSheet
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|Kit components||NADP/NADPH Extraction Buffer NADP Cycling Buffer NADP Cycling Enzyme Mix NADPH Developer Stop Solution NADPH Standard (MW:833.36)|