NADP/NADPH Quantitation Colorimetric Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nicotinamide adenine dinucleotide phosphate (NADP+) is a cofactor in the lipid and nucleic acid biosynthesis, which require NADPH as a reducing agent. NADP+ plays important roles in energy transforming and redox state of cells or tissues.
The NADP/NADPH Quantitation Colorimetric Kit provides a sensitive and convenient way for detection of the intracellular nucleotides: NADP, NADPH and their ratio. The NADP Cycling Enzyme Mix specifically recognize NADP/NADPH in an enzyme cycling reaction, which are not required to purify from samples. The enzyme cycling reaction significantly increases the detection specificity and sensitivity. Results can be easily quantified using plate reader at OD450 nm. The assay specifically detects NADP and NADPH, but not NAD or NADH.
| Features | Fast and convenient Simple procedure; takes ~2 hours. Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio. Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD+/NADH) |
| Shipping | Gel pack |
| Storage Conditions | Store at -20°C. |
| Kit components | NADP/NADPH Extraction Buffer NADP Cycling Buffer NADP Cycling Enzyme Mix NADPH Developer Stop Solution NADPH Standard (MW:833.36) |