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Folinic acid

Catalog No.
B7886
A folate analog; an adjuvant used in combination with the chemotherapy drugs
Grouped product items
SizePriceStock Qty
10mg
$50.00
In stock
50mg
$100.00
In stock

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Email: [email protected]

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Background

Folinic acid, clinically known as leucovorin, is a ubiquitous member of biological folate pools, usually accounting for 10 ~ 20% of total cellular folates. Folinic acid can reduce methotrexate (MTX)-induced cytogenetic damage, and appears to be an anticlastogenic agent. Folinic acid serves as a rescue agent used alone or in combination with 5-fluorouracil to decrease MTX-induced toxicity. 

References:

1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228.

2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt473.44
Cas No.58-05-9
FormulaC20H23N7O7
Solubility≥7.62 mg/mL in DMSO with gentle warming; insoluble in H2O; insoluble in EtOH
Chemical Name(2S)-2-(4-(((5-formyl-4-hydroxy-2-imino-1,2,5,6,7,8-hexahydropteridin-6-yl)methyl)amino)benzamido)pentanedioic acid
SDFDownload SDF
Canonical SMILESN=C1NC(NCC(N2C=O)([H])CNC3=CC=C(C(N[[email protected]@](C(O)=O)([H])CCC(O)=O)=O)C=C3)=C2C(O)=N1
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment:[1]

Cell lines

V79 cells

Reaction Conditions

5 or 50 μg/ml folinic acid for 40 h incubation

Applications

MTX alone induced a concentration-related increase in the percentages of micronucleated binucleated cells (MNBN) and aberrant cells (Abs). The addition of folinic acid at 50 μg/ml significantly reduced MNBN by 40 ~ 68% and Abs by 36 ~ 77%. Inhibition was also seen at 5 μg/ml folinic acid (12 to 54% for MNBN and 20 to 61% for Abs).

Animal experiment:[2]

Animal models

Balb/c young growing male mice, 11.9 ± 0.25 g

Dosage form

7.0 mg/kg

Injected intraperitoneally every second day for 3 weeks

Applications

Chronic administration of MTX induced suppression of skeletal growth in mice. Folinic acid treatment following MTX administration appeared to reverse this growth inhibition.

Note

The technical data provided above is for reference only.

References:

1. Keshava C, Keshava N, Whong WZ, et al. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutation Research, 1998, 397(2): 221-228.

2. Iqbal MP, Ahmed M, Umer M, et al. Effect of methotrexate and folinic acid on skeletal growth in mice. Acta Paediatrica, 2003, 92(12): 1438-1444.

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