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Caspase-4 Colorimetric Assay Kit

Catalog No.
K2199
Convenient, Highly-Sensitive Assay.
Grouped product items
SizePriceStock Qty
25 assays
$186.00
In stock
100 assays
$438.00
In stock
200 assays
$594.00
In stock
400 assays
$918.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Caspase-4 belongs to the caspase-family of cysteine proteases. Caspase-4 is an enzyme that proteolytically cleaves other proteins at an aspartic acid residue. Caspase-4 exists in cells as an inactive proenzyme. It is matured by proteolysis. The active Caspase-4 is a heterotetramer contain two large and two small subunits. The function of Caspase 4 is believed to be an inflammatory caspase, together with Caspase-1, Caspase-5, with a role in the immune system.

Apoptosis in mammalian cells is caused by activation of ICE-family proteases/caspases. The Caspase-4 Fluorometric Assay Kit provides a fast and simple means to assay the activity of caspases that recognize the sequence LEVD. The assay is according to spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. Using a spectrophotometer or a microtiter plate reader at 400 or 405 nm can quantify the pNA light emission. Comparison of the absorbance of pNA from an apoptotic sample with an un-induced control allows determination of the fold increase in LEVD-dependent caspase activity.

Quality Control

Quality Control & DataSheet

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Overview

Kit componentsCell Lysis Buffer, 2X Reaction Buffer, LEVD-pNA (4 mM), DTT (1 M), Dilution Buffer
Detection methodAbsorbance (400 or 405 nm)
Sample typeCell and tissue lysates

Features & Properties

FeaturesFast and convenient; Simple one-step procedure; Takes only 1-2 hours; Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity.
ShippingGel pack.
Storage ConditionsStore at -20°C.