Labeling of DNA/cDNA via PCR and functions as a reactive site for subsequent labeling by reactive fluorescent probes.
Sample solution is provided at 25 µL, 10mM.
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|Cas No.||116840-18-7||SDF||Download SDF|
|Synonyms||5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate,aa-dutp ,aa dutp|
|Chemical Name||((2R,3S,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate|
|Canonical SMILES||O=C(N1)N([[email protected]@H]2O[[email protected]](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[[email protected]@H](O)C2)C=C(/C=C/CN)C1=O|
|Formula||C12H20N3O14P3(free acid)||M.Wt||523.22(free acid)|
|Solubility||Soluble in sterile water||Storage||Store at -20°C|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
Aminoallyl-dUTP is used for indirect stable labeling of DNA/cDNA by polymerase chain reaction (PCR) with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, Primer Extension with Klenow exo-, 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) and Reverse Transcription with MMLV Reverse Transcriptase. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks or introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging and dot blots (cDNA probes) .
Aminoallyl-dUTP can also be introduced to probe by PCR and functions as a reactive site for subsequent labeling of the probe. The fluorescent labeling probe is used for in situ hybridization .
 Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.
 Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.