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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aminoallyl-dUTP is used for indirect stable labeling of DNA/cDNA by polymerase chain reaction (PCR) with Taq polymerase, Nick Translation with DNAse I/ DNA Polymerase I, Primer Extension with Klenow exo-, 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) and Reverse Transcription with MMLV Reverse Transcriptase. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Amine-functionalized DNA/cDNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces a Biotin group (via NHS Ester of Biotin) for subsequent purification tasks or introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging and dot blots (cDNA probes) [1][2].
Aminoallyl-dUTP can also be introduced to probe by PCR and functions as a reactive site for subsequent labeling of the probe. The fluorescent labeling probe is used for in situ hybridization [2].
References:[1] Dirsch et al. (2007) Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes. Appl. Immunohistochem. Mol. Morphol. 3:332.[2] Cox et al. (2004) Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling. BioTechniques 36:114.