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8-Bromo-cAMP, sodium salt

In stock
Catalog No.
B9000
Cell-permeable cAMP analog that activates PKA
Grouped product items
SizePriceStock Qty
10mg
$48.00
In stock
25mg
$88.00
In stock

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8-Bromo-cAMP is a cAMP-analogues. [1]

cAMP can have an effect on the proliferative response of a cell. Either the effect is a positive or a negative regulator depending on the cell types. There is also variable effect of cAMP in hematopoietic cell lines. CAMP-analogues can significantly inhibit the erythropoietin-stimulated proliferation.[1]

8-Bromo-cAMP increased the cellular content of mRNA encoding the hCG a- and β-subunits and prevented the increase in fibronectin mRNA. This is determined by blot hybridization analysis using specific cDNA probes. 8-Bromo-cAMP also induced phosphorylation of Erk1,2 in AML193 cells. 8-Bromo-cAMP is an agent in AML193 cells and activates Erk1,2 , this condition happens does not accompanied by the involvement of Shc phosphorylation.[1,2]

8-bromo-cAMP increases catecholamine biosynthesis and produces an increase both in the activity and phosphorylation of tyrosine hydroxylase. 8-bromo-cAMP also promotes alterations in the synthesis and secretion of specific proteins, including fibronectin and the subunits of hCG by regulating mRNA expression.[2,3]

References:
[1]Ulloa-Aguirre A,?August AM,?etal. , 8-Bromo-adenosine?3',5'-monophosphate?regulates?expression?of?chorionic?gonadotropin?and fibronectin in human cytotrophoblasts. J Clin Endocrinol Metab.?1987 May;64(5):1002-9.
[2] Rene e M. Y. Barge, J.H. Frederik Falkenburg, etal., 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk1,2 via a Shc-independent pathway. Biochimica et Biophysica Acta 1355 (1997) 141–146.
[3] Haycock JW, Bennett WF, etal. , Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem. 1982 Nov 25;257(22):13699-703.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt430.09
Cas No.76939-46-3
FormulaC10H10BrN5NaO6P
Solubility≥43mg/mL in H2O
Chemical Namesodium (4aR,6R,7R,7aS)-6-(6-amino-8-bromo-9H-purin-9-yl)-7-hydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide
SDFDownload SDF
Canonical SMILESNC1=C(N=C(Br)N2[[email protected]@H]3O[[email protected]](COP(O4)([O-])=O)[[email protected]@H]4[[email protected]]3O)C2=NC=N1.[Na+]
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell experiment [1]:

Cell lines

Growth-arrested AML193 cells

Preparation method

The solubility of this compound in sterile water is 100 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 15 μM

Applications

In growth-arrested AML193 cells, 8-Bromo-cAMP significantly increased DNA synthesis, with the ED50 value of approximately 10 μM. At the doses over 2.5 μM, a toxic effect was observed. Moreover, the addition of 1 μM 8-Bromo-cAMP synergized the IL-3-induced mitogenic response. In addition, 8-Bromo-cAMP at the concentration of 1μM resulted in maximal Erk phosphorylation. But the effect of 8-Bromo-cAMP on Erk phosphorylation was transient, with the maximal stimulation shown between 1 and 5 mins.

References:

[1]. Rene e M. Y. Barge, J.H. Frederik Falkenburg, etal., 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk1,2 via a Shc-independent pathway. Biochimica et Biophysica Acta 1355 (1997) 141–146.

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