8-Bromo-cAMP, sodium salt
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
8-Bromo-cAMP is a cAMP-analogues. 
cAMP can have an effect on the proliferative response of a cell. Either the effect is a positive or a negative regulator depending on the cell types. There is also variable effect of cAMP in hematopoietic cell lines. CAMP-analogues can significantly inhibit the erythropoietin-stimulated proliferation.
8-Bromo-cAMP increased the cellular content of mRNA encoding the hCG a- and β-subunits and prevented the increase in fibronectin mRNA. This is determined by blot hybridization analysis using specific cDNA probes. 8-Bromo-cAMP also induced phosphorylation of Erk1,2 in AML193 cells. 8-Bromo-cAMP is an agent in AML193 cells and activates Erk1,2 , this condition happens does not accompanied by the involvement of Shc phosphorylation.[1,2]
8-bromo-cAMP increases catecholamine biosynthesis and produces an increase both in the activity and phosphorylation of tyrosine hydroxylase. 8-bromo-cAMP also promotes alterations in the synthesis and secretion of specific proteins, including fibronectin and the subunits of hCG by regulating mRNA expression.[2,3]
Ulloa-Aguirre A,?August AM,?etal. , 8-Bromo-adenosine?3',5'-monophosphate?regulates?expression?of?chorionic?gonadotropin?and fibronectin in human cytotrophoblasts. J Clin Endocrinol Metab.?1987 May;64(5):1002-9.
 Rene e M. Y. Barge, J.H. Frederik Falkenburg, etal., 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk1,2 via a Shc-independent pathway. Biochimica et Biophysica Acta 1355 (1997) 141–146.
 Haycock JW, Bennett WF, etal. , Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem. 1982 Nov 25;257(22):13699-703.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥43mg/mL in H2O|
|Chemical Name||sodium (4aR,6R,7R,7aS)-6-(6-amino-8-bromo-9H-purin-9-yl)-7-hydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide|
|Canonical SMILES||NC1=C(N=C(Br)N2[[email protected]@H]3O[[email protected]](COP(O4)([O-])=O)[[email protected]@H]4[[email protected]]3O)C2=NC=N1.[Na+]|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Cell experiment :|
Growth-arrested AML193 cells
The solubility of this compound in sterile water is 100 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.
0 ~ 15 μM
In growth-arrested AML193 cells, 8-Bromo-cAMP significantly increased DNA synthesis, with the ED50 value of approximately 10 μM. At the doses over 2.5 μM, a toxic effect was observed. Moreover, the addition of 1 μM 8-Bromo-cAMP synergized the IL-3-induced mitogenic response. In addition, 8-Bromo-cAMP at the concentration of 1μM resulted in maximal Erk phosphorylation. But the effect of 8-Bromo-cAMP on Erk phosphorylation was transient, with the maximal stimulation shown between 1 and 5 mins.
. Rene e M. Y. Barge, J.H. Frederik Falkenburg, etal., 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk1,2 via a Shc-independent pathway. Biochimica et Biophysica Acta 1355 (1997) 141–146.
Quality Control & MSDS
- View current batch:
Purity = 99.07%
- COA (Certificate Of Analysis)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)