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Tn5 DNA Library Prep Kit for Illumina (for 1 ng DNA)

Catalog No.
K1055
Direct and rapid library construction of purified DNA samples by transposase
Grouped product items
SizePriceStock Qty
24 rxns
$558.00
In stock
96 rxns
$2,160.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Quality Control

Quality Control & DataSheet

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Components and Storage

Components 24 rxns 96 rxns
Transposase Mix 120 uL 480 uL
2xTagmetation Buffer 240 uL 960 uL
5xStop Solution 120 uL 480 uL
Amplify Enzyme 24 uL 96 uL
5xAmplify Buffer 240 uL 960 uL
dNTP 96 uL 384 uL
Control DNA 10 uL 10 uL

Control DNA, Escherichia coli Genomic DNA, 50 ng/uL
Store 5xStop Solution at room temperature, Transposase Mix at 4°C and other components at -20°C

Description

Tn5 DNA Library Prep Kit for Illumina (for 1 ng DNA) is an optimized genomic library construction kit for the Illumina high-throughput sequencing platform, which is suitable for the amount of 1 ng input DNA. Traditional library construction generally includes multi-step operations such as DNA fragmentation, end repair and linker ligation, while our kit adopts Tn5 transposase method which can be completed in one-step reaction. Our kits are easy to operate and greatly reduce library construction time, and significantly reducing the demanded amount of the initial DNA.

The Transposase Mix and buffers provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.

The kit is suitable for a variety of purified DNA samples including human, animal, plant, microbial genomes and PCR products. When using PCR products for library preparation, make sure the size of PCR products is > 500 bp. The transposase is not effective at the ends of DNA, to avoid the reduction of sequencing coverage on the ends, it is recommended to extend 50-100 bp at the both ends of the to-be-sequenced region when PCR products are prepared.

Overview of the Workflow

Features

1. One tube enzymatic reaction, easy to operate

2. A single sample library process takes only 90 minutes

3. Sample input DNA volume can be as low as 1 ng, high library conversion efficiency

4. The Transposase Mix and buffers provided by the kit are subject to rigorous quality control and functional verification, so as to achieve high fidelity, stability and repeatability.