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Cyanine dyes

Cyanine dyes are molecules containing polymethine bridges between two nitrogen atoms with a delocalizedcyanine_dyes charge which have been used for many years as dyes in life science. Since the Cy3, Cy5 and Cy7 were made commercially available as succinimidyl esters in the early 1990s, cyanine dyes began to be used widely as labels for nucleic acids. Because of the good properties of low non-specific binding to biomolecules and bright fluorescence owing to their huge extinction coefficients and good quantum yields, the Cy dyes have been widespread used in DNA and RNA labelling. Cyanine dyes can be divided into two groups based on water solubility: non-sulfonated cyanines and sulfonated cyanines.

Non-sulfonated cyanines
Available non-sulfonated dyes include Cy3, Cy3.5, Cy5, Cy5.5, Cy7, and Cy7.5. Cy stands for 'cyanine', and the first digit identifies the number of carbon atoms between the indolenine groups. The suffix .5 is added for benzo-fused cyanines. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. They are organic co-solvent soluble (5-20% of DMF or DMSO). When these molecules are used for biomolecule labeling, they should be dissolved in organic solvent first, and added to a solution of biomolecule (protein, peptide, amino-labeled DNA) in appropriate aqueous buffer. Fluorescent properties of non-sulfonated cyanines have little dependence on solvent and surrounding.

Sulfonated cyanines
Available sulfonated cyanines include sulfo-Cy3, sulfo-Cy5, and sulfo-Cy7, which have additional sulfo-groups that facilitate dissolution of dye molecules in aqueous phase. Charged sulfonate groups decrease aggregation of dye molecules and heavily labeled conjugates. Sulfonated cyanines are highly water soluble, and they do not use organic co-solvent for the labeling in aqueous environment.

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  1. Cy5 carboxylic acid (non-sulfonated)
    Catalog No. :
    A8133
    Non-reactive fluorophore, for control experiments, and for calibration
    Non-activated carboxylic acid. Contains Cy5 fluorophore.
  2. Cy3 carboxylic acid (non-sulfonated)
    Catalog No. :
    A8132
    Non-reactive fluorophore, for control experiments, and for calibration
    Free Cy3 carboxylic acid, non-activated dye. Non-sulfonated reagent, with good solubility in organic solvents, and limited aquous solubility.
  3. Cy7.5 azide (non-sulfonated)
    Catalog No. :
    A8116
    used for Click chemistry to label alkyne-modified oligonucleotides
    Cy7.5 is a NIR dye with long-wave infrared fluorescence. This derivative is azide for Click Chemistry.
  4. Cy7 azide (non-sulfonated)
    Catalog No. :
    A8115
    used for Click chemistry to label alkyne-modified oligonucleotides
    Cy7 azide is a near-infrared fluorescent dye azide for Click Chemistry labeling.
  5. Cy5 alkyne (non-sulfonated)
    Catalog No. :
    A8131
    used for Click chemistry to label any molecule bearing azide group.
    Cy5 alkyne for Click Chemistry. With this product, deeply colored, and photostable Cy5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.
  6. Cy5.5 azide (non-sulfonated)
    Catalog No. :
    A8114
    used for Click chemistry to label alkyne-modified oligonucleotides
    This Cy5.5 labeling reagent is a dye azide for Click Chemistry, available as 10 mM solution in DMSO, or in solid form.
  7. Cy5 azide (non-sulfonated)
    Catalog No. :
    A8113
    used for Click chemistry to label alkyne-modified oligonucleotides
    Cy5 azide labeling reagent for Click Chemistry, available as 10 mM solution in DMSO, and in solid form.
  8. Cy3 alkyne (non-sulfonated)
    Catalog No. :
    A8130
    used for Click chemistry to label any molecule bearing azide group.
    Cy3 alkyne is a fluorophore ready for the use in Click Chemistry reaction.
  9. Cy3.5 azide (non-sulfonated)
    Catalog No. :
    A8112
    used for Click chemistry to label alkyne-modified oligonucleotides
    Cy3.5 labeling reagent, solid material and 10 mM solution in DMSO, for Click Chemistry.
  10. Cy3 azide (non-sulfonated)
    Catalog No. :
    A8111
    used for Click chemistry to label alkyne-modified oligonucleotides
    Cy3 dye azide for Click Chemistry.

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