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Cy3.5 hydrazide (non-sulfonated) carbonyl-reactive dye

Catalog No.A8266
Size Price Stock Qty
Cy3.5 hydrazide 1mg
$110.00
In stock
Cy3.5 hydrazide 5mg
$210.00
In stock
Cy3.5 hydrazide 25mg
$410.00
In stock
Cy3.5 hydrazide 50mg
$695.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & MSDS

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Chemical structure

Cy3.5 hydrazide (non-sulfonated)

Cy3.5 hydrazide (non-sulfonated) Dilution Calculator

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Cy3.5 hydrazide (non-sulfonated) Molarity Calculator

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Chemical Properties

Cas No. N/A SDF Download SDF
Chemical Name 3-(6-hydrazinyl-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium chloride hydrochloride
Canonical SMILES CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C4=[N+](CCCCCC(NN)=O)C5=CC=C(C=CC=C6)C6=C5C4(C)C.Cl.[Cl-]
Formula C38H44Cl2N4O M.Wt 643.69
Solubility >64.4mg/mL in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Spectral properties

Excitation max (nm) 591 Emission max (nm) 604
Extinction Coefficient (M-1cm-1) 116000 Quantum Yield 0.35
CF260 0.29 CF280 0.22

Application

Non-sulfonated cyanines are available for the labeling of many targets including:

• Soluble proteins, which are tolerant to addition of organic co-solvent • Fluorescence polarization (FP)
• Antibodies (with 5-10% of DMSO/DMF) • Fluorescence resonance energy transfer (FRET)
• DNA and oligonucleotides • Time-resolved fluorescence energy transfer (TR-FRET)
• Peptides • Fluorescence intensity (FI)
• Many small molecules  
• Reactions in organic media (dichloromethane, acetonitrile)  

Background

Cy3.5 hydrazide is a near infrared fluorophore that can react with various carbonyl groups in biomolecules which are generated after oxidative stress or deamination of proteins. In addition, aldehyde moieties can be produced by periodate oxidation of sugar residues of glycoproteins, such as antibodies that can then be oxidized by sodium periodate and labeled with Cy3.5 hydrazide to form Cy3. 5 labeled antibodies. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.