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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
• Protein labeling—biotin antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes • Antibody labeling—biotin antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes • Cell surface labeling—biotin only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes • Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides • Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds • Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved • Medium length—spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Sulfo-NHS-LC-Biotin is one of three very similar Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-LC-Biotin is offered in several package sizes and as complete protein labeling kits.