C-176

C-176 (CAS No.: 314054-00-7) is a selective, covalent small-molecule inhibitor of the stimulator of interferon genes (STING) pathway with demonstrated blood-brain barrier permeability, making it a valuable tool for investigating innate immune signaling in both peripheral and central nervous system contexts. Mechanistically, C-176 targets a critical cysteine residue within the transmembrane domain of STING, thereby preventing activation-induced palmitoylation and subsequent downstream signaling, leading to suppression of type I interferon production and interferon-stimulated gene expression. In cellular systems, it selectively inhibits STING-mediated IFN-β reporter activity without significantly affecting parallel pathways such as RIG-I or TBK1, with functional activity observed across nanomolar to low micromolar concentrations. In vivo, C-176 has been shown to attenuate systemic interferon responses and inflammatory pathology in murine models of STING-driven disease, supporting its utility in studying autoinflammatory disorders and immune dysregulation. This compound is widely applied in cell-based assays and animal studies to dissect STING-dependent signaling mechanisms, neuroinflammation, and host defense pathways, and it serves as a reference inhibitor in drug discovery efforts targeting aberrant STING activation, with experimental concentrations or dosing regimens tailored to specific study designs.