HyperScribe™ Co-transcription mRNA Synthesis Kit Plus (ARCA, 5mCTP, ψUTP, T7)
HyperScribe™ Co-transcription mRNA Synthesis Kit plus (ARCA, 5mCTP, ψUTP, T7) is designed for quick production of ARCA capped mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) and ψUTP(Catalog No. B7972) can reduce host cell immune response. In addition, Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. This kit recommends using a DNA template with a tailed sequence at the 3' end for producting mRNA with stable poly(A) tail (typically 100-120 A).
RNA synthesized using this kit has many applications in biological experiments, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25 reactions, 20 μL each time. It adjusts the composition and reaction based on K1064 to achieve higher RNA yields for standard 20 μL reaction systems.
| Components | 25 rxn |
| T7 RNA Polymerase Mix | 50 μL |
| 10 × Reaction Buffer | 50 μL |
| ATP (100 mM) | 30 μL |
| Pseudo-UTP (100 mM) | 25 μL |
| 5mCTP (100 mM) | 25 μL |
| GTP (100 mM) | 25 μL |
| ARCA (100 mM) | 20 μL |
| Control Template (0.5 μg/μL) | 5 μL |
| RNase-free H2O | 0.5 mL |
Store the components at -20°C. | |