2-Deoxy-D-glucose
2-Deoxy-D-glucose (2-DG)(CAS 154-17-6) is a glucose analog that acts as a competitive inhibitor of glycolysis, interfering with cellular glucose metabolism and ATP synthesis. It suppresses glycolytic flux, altering cellular energy levels and inducing metabolic stress. In vitro studies demonstrate its cytotoxicity against the KIT-positive gastrointestinal stromal tumor (GIST) cell lines GIST882 and GIST430, with IC50 values of 0.5 μM and 2.5 μM, respectively. Additionally, treatment with 2-DG impairs viral protein translation during early virus replication stages and can potentiate chemotherapeutic agents' cytotoxicity in human cancer models, supporting its applicability in metabolic pathway studies and cancer therapy research.
References:
[1]. John Laszlo, William R. Harlan, Robert F. Klein, et al. The Effect of 2-Deoxy-D-Glucose Infusions on Lipid and Carbohydrate Metabolism in Man. J Clin Invest., 1961, 40(1):171-176.
[2]. Andrean L. Simons, Iman M. Ahmad, David M. Mattson, et al. 2-Deoxy-D-Glucose Combined with Cisplatin Enhances Cytotoxicity via Metabolic Oxidative Stress in Human Head and NeckCancer Cells. Cancer Research, 2007, 67(7): 3364-3371.
[3]. Gregory Maschek, Niramol Savaraj, Waldemar Priebe, et al. 2-Deoxy-D-glucose Increases the Efficacy of Adriamycin and Paclitaxel in Human Osteosarcoma and Non-Small Cell Lung Cancers In Vivo. Cancer Research, 2004, 64:31-34.
[4]. Thomas Mühlenberg, Susanne Grunewald, Jürgen Treckmann, et al. Inhibition of KIT-Glycosylation by 2-Deoxyglucose Abrogates KIT-Signaling and Combination with ABT-263 Synergistically Induces Apoptosis in Gastrointestinal Stromal Tumor. PLOS ONE, 2015, 10(3):e0120531.
[5]. R. J. Connett, C. R. Honig, T. E. Gayeski, et al. Defining hypoxia: a systems view of VO2, glycolysis, energetics, and intracellular PO2. Journal of Applied Physiology, 1990, 68(3): 833-842.
[6]. Yue Wang, Jia-rong Li, Ming-xia Sun, et al. Triggering unfolded protein response by 2-Deoxy-D-glucose inhibits porcine epidemic diarrhea virus propagation. Antiviral Research, 2014, 106: 33–41.
[7]. F.A. Guardiola, R. Cerezuela, J. Meseguer, et al. Effects of 2-deoxy-D-glucose on the immune system of seabream (Sparus aurata L.). Fish & Shellfish Immunology, 2011, 30: 592-599.
- 1. Qinling Pan, Tengteng Xin, et al. "Metabolic immune regulation of macrophages by melanized fungus Fonsecaea monophora." Available online 28 July 2025, 101571
- 2. Dan Li, Jing Yang, et al. "Enterovirus A71-induced glycolysis is essential for viral replication by activating PI3K/Akt pathway." Microb Pathog. 2025 Jun:203:107505. PMID: 40127828
- 3. Zhongjun Wan, Shanshan Wen, et al. "Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells." STAR Protoc. 2025 Mar 21;6(1):103576. PMID: 39893639
- 4. Jiawei Yan, Xin Zhang, et al. "Macrophage NRF1 promotes mitochondrial protein turnover via the ubiquitin proteasome system to limit mitochondrial stress and inflammation." Cell Rep. 2024 Sep 25;43(10):114780 PMID: 39325625
- 5. Chengjia You, Fangyuan Shen, et al. "O-GlcNAcylation mediates Wnt-stimulated bone formation by rewiring aerobic glycolysis." EMBO Rep. 2024 Sep 10. PMID: 39256595
- 6. Lei Li, Shuangshuang Sun, et al. "Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation." Research Square. 26 Sep, 2024
- 7. Jun Xiao, Shuang Wang, et al. "25-Hydroxycholesterol regulates lysosome AMP kinase activation and metabolic reprogramming to educate immunosuppressive macrophages." Immunity. 2024 May 14;57(5):1087-1104.e7. PMID: 38640930
- 8. Wanli Liu, et al. "MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation." Nat Commun. 2024 Jan 2;15(1):163. PMID: 38167945
- 9. Méghane Sittewelle, Stephen J Royle, et al. "Passive diffusion accounts for the majority of intracellular nanovesicle transport." Life Sci Alliance. 2023 Oct 19;7(1):e202302406. PMID: 37857498
- 10. Ren He, Xiaohan Li, et al. "Dexamethasone inhibits IL-8 via glycolysis and mitochondria-related pathway to regulate inflammatory pain." BMC Anesthesiol. 2023 Sep 18;23(1):317. PMID: 37723417
- 11. Xiaofeng Li, Yiwen Chen, et al. "GPR81-mediated reprogramming of glucose metabolism contributes to the immune landscape in breast cancer." Discov Oncol. 2023 Jul 27;14(1):140. PMID: 37500811
- 12. Xu-Zhe Fu, Yu Wang, et al. "Interferon-γ regulates immunosuppression in septic mice by promoting the Warburg effect through the PI3K/AKT/mTOR pathway." Mol Med. 2023 Jul 11;29(1):95. PMID: 37434129
- 13. Yiman He, Huawan Chen, et al. "3-Bromopyruvate-loaded bismuth sulfide nanospheres improve cancer treatment by synergizing radiotherapy with modulation of tumor metabolism." J Nanobiotechnology. 2023 Jul 5;21(1):209. PMID: 37408010
- 14. Joshua Saliutama. "Fatty Acids and Parasitism: Towards a Better Understanding of Lipid Metabolism in Trypanosoma Brucei." Clemson University. August 2023.
- 15. Ye Liu, Wenna Chi, et al. "Ablation of Proton/glucose Exporter SLC45A2 Enhances Melanosomal Glycolysis to Inhibit Melanin Biosynthesis and Promote Melanoma Metastasis." J Invest Dermatol. 2022 Apr 23;S0022-202X(22)00301-3. PMID: 35469906
- 16. Fang Wang, Jing Zhang, et al. "2-Deoxy-D-glucose impedes T cell-induced apoptosis of keratinocytes in oral lichen planus." J Cell Mol Med. 2021 Nov;25(21):10257-10267. PMID: 34672419
- 17. Beatriz Lapa, Ana Cristina Gonçalves, et al. "Acute myeloid leukemia sensitivity to metabolic inhibitors: glycolysis showed to be a better therapeutic target." Med Oncol. 2020 Jul 28;37(8):72. PMID: 32725458
- 18. Wang F, Zhang J, et al. "HIF1α/PLD2 axis linked to glycolysis induces T-cell immunity in oral lichen planus." Biochim Biophys Acta Gen Subj. 2020;1864(7):129602. PMID: 32205175
- 19. Wang F, Zhang J, et al. "The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus." Immunobiology. 2020;225(3):151933. PMID: 32201095
- 20. Li X, Zhang Y, et al. "Enhanced glucose metabolism mediated by CD147 contributes to immunosuppression in hepatocellular carcinoma." Cancer Immunol Immunother. 2020;10.1007/s00262-019-02457-y. PMID: 31965268
- 21. Barot S, Abo-Ali EM, et al. "Inhibition of glycogen catabolism induces intrinsic apoptosis and augments multikinase inhibitors in hepatocellular carcinoma cells." Exp Cell Res. 2019 Aug 15;381(2):288-300. PMID: 31128107
- 22. Tian C, Yuan Z, et al. "Inhibition of glycolysis by a novel EGFR/HER2 inhibitor KU004 suppresses the growth of HER2+ cancer." Exp Cell Res. 2017 May 19. pii: S0014-4827(17)30297-5. PMID: 28532652
| Storage | Store at -20°C |
| M.Wt | 164.16 |
| Cas No. | 154-17-6 |
| Formula | C6H12O5 |
| Synonyms | 2-DG |
| Solubility | ≥105 mg/mL in H2O; ≥2.37 mg/mL in EtOH with gentle warming and ultrasonic; ≥8.2 mg/mL in DMSO |
| Chemical Name | (4R,5S,6R)-6-(hydroxymethyl)tetrahydro-2H-pyran-2,4,5-triol |
| SDF | Download SDF |
| Canonical SMILES | [H][C@]([C@](C1([H])[H])([H])O[H])([C@](C([H])([H])O[H])([H])OC1([H])O[H])O[H] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1, 2]: | |
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Cell lines |
GIST cell lines, Vero cells infected with PEDV |
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Preparation method |
The solubility of this compound in DMSO is >8.2 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
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Reacting condition |
5 mM, 10 mM, 24 h |
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Applications |
2DG dose-dependent accumulation of cells in G1 phase and reduction of S phase cells with the IC50 values between 0.5 μM and 2.5 μM. 2-DG inhibited PEDV replication and gene expression in Vero cells. 2-DG (10 mM for 24 h) treatment affected virus packaging. |
| Animal experiment [3]: | |
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Animal models |
Nude mouse xenograft models of human osteosarcoma and non-small cell lung cancer |
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Dosage form |
500 mg/kg, i.p., 3 × per week (Monday, Wednesday, and Friday) |
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Application |
ADR (6 mg/kg, i.v.) + 2-DG (500 mg/kg, i.p.) combination treatment resulted in significant slower tumor growth than 2-DG alone. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Mühlenberg T, Grunewald S, Treckmann J, et al. Inhibition of KIT-glycosylation by 2-deoxyglucose abrogates KIT-signaling and combination with ABT-263 synergistically induces apoptosis in gastrointestinal stromal tumor[J]. PloS one, 2015, 10(3): e0120531. [2]. Wang Y, Li J, Sun M, et al. Triggering unfolded protein response by 2-Deoxy-D-glucose inhibits porcine epidemic diarrhea virus propagation[J]. Antiviral research, 2014, 106: 33-41. [3]. Gregory Maschek, Niramol Savaraj, Waldemar Priebe, et al. 2-Deoxy-D-glucose Increases the Efficacy of Adriamycin and Paclitaxel in Human Osteosarcoma and Non-Small Cell Lung Cancers In Vivo. Cancer Research, 2004, 64:31-34. |
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Quality Control & MSDS
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