Z-DEVD-pNA
Z-DEVD-pNA is a colorimetric substrate for caspase-3 with increased cell permeability. Sequence is based on PARP poly(ADP-ribose) polymerase which is clevaved by caspase-3 at the amino acid sequence DEVD[1]. Z-DEVD-pNA also recognizes caspases-1, -4 and -7. The N-terminus of Z-DEVD-pNA is blocked by a benzyloxycarbonyl (Z) moiety that could increase cell permeability. The Caspase-3 activity is calculated by sepctrophotometric reading of free pNA (l = 400 nm) following cleavage from the substrate DEVD-pNA. [2] Z-DEVD-pNA can be used to identify and quantify caspase-3 activity in apoptotic cell lysates.
References:
1. Lazebnik, Y.A.,Kaufmann, S.H.,Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371, 346-347 (1994).
2. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis: D.W. Nicholson, et al.; Nature 376, 37 (1995)
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 730.68 |
| Formula | C32H38N6O14 |
| Solubility | ≥28.35 mg/mL in DMSO; ≥4.31 mg/mL in EtOH with ultrasonic; ≥7.31 mg/mL in H2O |
| Chemical Name | 8-(2-carboxyethyl)-5-(carboxymethyl)-11-isopropyl-14-((4-nitrophenyl)carbamoyl)-3,6,9,12-tetraoxo-1-phenyl-2-oxa-4,7,10,13-tetraazahexadecan-16-oic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(NC(C(NC(C(NC(C(NC(CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)C(C)C)=O)CCC(O)=O)=O)CC(O)=O)OCC2=CC=CC=C2 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
